| 逆转录病毒介导IL-27基因对胰腺癌细胞在裸鼠体内的成瘤抑制作用及其机制 |
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| 引用本文： | 刘丽华,艾军,孟君,张超,赵连梅,邵丽丽,单保恩. 逆转录病毒介导IL-27基因对胰腺癌细胞在裸鼠体内的成瘤抑制作用及其机制[J]. 中国免疫学杂志, 2010, 26(6). DOI: 10.3969/j.issn.1000-484X.2010.06.005 |
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| 作者姓名： | 刘丽华 艾军 孟君 张超 赵连梅 邵丽丽 单保恩 |
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| 作者单位： | 河北医科大学第四医院科研中心/河北省肿瘤研究所,石家庄,050011 |
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| 基金项目： | 河北省科技厅科研基金项目 |
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| 摘 要： | 目的:探讨IL-27基因在人胰腺癌Aspc1细胞中的抗肿瘤作用及免疫机制。方法:以逆转录病毒为载体,采用基因转染的方法用G418梯度筛选法建立转染IL-27基因的Aspc1细胞,用RT-PCR检测其基因导入,ELISA法检测IL-27的分泌。将Aspc1/IL-27、Aspc1/LXSN和Aspc1细胞接种于裸鼠皮下,观察成瘤性、移植瘤的生长情况。10天时取3组裸鼠脾脏,用ELISA法检测3组小鼠脾细胞在Aspc1诱导下IFN-γ、TNF-α和IL-12的产生情况,乳酸脱氢酶法检测脾细胞杀伤活性。结果:成功建立稳定转染的Aspc1/IL-27细胞株,ELISA检测Aspc1/IL-27细胞培养上清中IL-27的分泌量为(121.56±6.29)pg/ml,而在Aspc1/LXSN细胞和Aspc1细胞的培养上清中未检出IL-27。IL-27基因转染组裸鼠皮下结节生长速度明显慢于接种空载体转染组及未转染组;接种Aspc1/IL-27细胞的裸鼠脾细胞可产生较水平的IFN-γ、TNF-α和IL-12等细胞因子,与接种Aspc1/LXSN细胞和Aspc1细胞组裸鼠的脾细胞相比明显增高(P0.01),接种Aspc1/IL-27细胞的裸鼠脾细胞杀伤活性显著性升高(P0.01)。结论:转染IL-27基因的裸鼠胰腺癌细胞所分泌的IL-27具有生物学活性,通过增强细胞免疫功能在裸鼠体内发挥抗肿瘤作用。
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| 关 键 词： | 裸鼠 抗肿瘤 |
The antitumor activity of IL-27 gene transduced into Aspc1 cells and its mechanism in nude mouse |
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| LIU Li-Hua,AI Jun,MENG Jun,ZHANG Chao,ZHAO Lian-Mei,SHAO Li-Li,SHAN Bao-En. The antitumor activity of IL-27 gene transduced into Aspc1 cells and its mechanism in nude mouse[J]. Chinese Journal of Immunology, 2010, 26(6). DOI: 10.3969/j.issn.1000-484X.2010.06.005 |
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| Authors: | LIU Li-Hua AI Jun MENG Jun ZHANG Chao ZHAO Lian-Mei SHAO Li-Li SHAN Bao-En |
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| Affiliation: | LIU Li-Hua,AI Jun,MENG Jun,ZHANG Chao,ZHAO Lian-Mei,SHAO Li-Li,SHAN Bao-En.Research Cencer of the Fourth Hospital of Hebei Medical University/Hebei Cancer Institute,Shijiazhuang 050011,China |
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| Abstract: | Objective:To investigate the effects of interleukin-27 gene expression in human pancreatic carcinoma cells (Aspc1) and the related mechanisms.Methods:The retrovirus vector was used to transduce IL-27 gene into Aspc1 cells and stable clones were obtained by selecting with G418.The expression of IL-27p28 and IL-27EBI3 in Aspc1/IL-27 cells was detected by RT-PCR.The amount of IL-27 secreted from Aspc1/IL-27 cells was determined by ELISA.Subcutaneous injected Aspc1/IL-27,Aspc1/LXSN and Aspc1 cells into nude mic... |
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| Keywords: | IL-27 Aspc1 |
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