| 碱性成纤维细胞生长因子对体外培养人牙髓细胞增殖和迁移的影响 |
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| 引用本文： | 韦永珍,王秀颖,潘乙怀,邓辉.碱性成纤维细胞生长因子对体外培养人牙髓细胞增殖和迁移的影响[J].现代口腔医学杂志,2011,25(2):126-130. |
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| 作者姓名： | 韦永珍 王秀颖 潘乙怀 邓辉 |
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| 作者单位： | 1. 温州医学院口腔学院,325027
2. 温州医学院附属口腔医院 |
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| 基金项目： | 浙江省教育厅科研项目(Y200907095); 浙江省高校科研计划项目(Y200803098); 浙江省大学生科技创新项目(18) |
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| 摘 要： | 目的研究碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）对体外培养人牙髓细胞增殖、迁移活性的影响。方法以常规组织块培养法体外获得人牙髓细胞,实验组分别加入终浓度为1.0、5.0、10.0和50.0ng/ml的bFGF,对照组仅加入等量的空白培养基,采用CCK-8法分别测定450nm下各组光吸收度A值,研究bFGF对牙髓细胞增殖活性的影响;应用Transwell小室培养法,研究bFGF对牙髓细胞迁移活性的影响。结果与对照组相比,bFGF在1.0～50.0ng/ml浓度下可促进牙髓细胞的增殖（P〈0.05）,bFGF最低显效浓度为1.0ng/ml,最佳显效浓度为10.0ng/ml。bFGF在1.0～50.0ng/ml浓度下诱导细胞迁移（P〈0.05）。结论 bFGF可诱导体外培养人牙髓细胞的增殖和迁移,在牙髓牙本质复合体修复中可能发挥重要作用。
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| 关 键 词： | 碱性成纤维细胞生长因子 人牙髓细胞 增殖 迁移 |
Effect of bFGF on the proliferation and migration of human dental pulp cells in vitro |
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| WEI Yongzhen,WANG Xiuying,PAN Yihuai.School of Stomatology,Wenzhou Medical College,Wenzhou.Effect of bFGF on the proliferation and migration of human dental pulp cells in vitro[J].Journal of Modern Stomatology,2011,25(2):126-130. |
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| Authors: | WEI Yongzhen WANG Xiuying PAN YihuaiSchool of Stomatology Wenzhou Medical College Wenzhou |
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| Institution: | WEI Yongzhen,WANG Xiuying,PAN Yihuai.School of Stomatology,Wenzhou Medical College,Wenzhou 325000 |
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| Abstract: | Objective To investigate the effect of basic fibroblast growth factor(bFGF) on the proliferation and migration of human dental pulp cells in vitro. Methods After being isolated from dental pulp tissue explants,the human dental pulp cells were divided into 5 groups randomly,and bFGF was added into each group until the ultimate concentrations were 1.0,5.0,10.0 and 50.0ng/ml respectively,while non-bFGF group served as control.The proliferation ability of dental pulp cells induced by bFGF at different concentra... |
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| Keywords: | Basic fibroblast growth factor Human dental pulp cells Proliferation Migration |
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