小鼠移植物抗宿主病模型的建立

背景:小鼠移植物抗宿主病是异基因造血干细胞移植的主要并发症,表现为多系统损害(皮肤、食管、胃肠、肝脏等)的全身性疾病,是造成异基因造血干细胞移植后死亡的重要原因之一。目的:探讨建立小鼠移植物抗宿主病模型的方法,为实验性研究药物防治移植物抗宿主病提供有效的动物模型。方法:选择C57BL/6(H-2b)→BALB/C(H-2d)作为完全异基因移植供、受体。通过注射移植供鼠脾脏淋巴细胞及骨髓细胞数以建立移植物抗宿主病模型。根据临床表现、病理检查等判断移植物抗宿主病模型是否建立。结果与结论:给予5×106个以上的供鼠脾细胞的小鼠都发生了急性移植物抗宿主病,但小鼠移植物抗宿主病出现的时间、存活期不同。提示,输入5×106个供鼠脾细胞可有效建立移植物抗宿主病模型,小鼠异基因骨髓移植动物模型是可靠的,可用于移植物抗宿主病防治的实验性研究。

Establishment of a mouse model of graft-versus-host disease

BACKGROUND:Mouse graft-versus-host disease (GVHD) is the main complication of allogenic hematopoietic stem cell transplantation,presents the damages to multisystem (skin,esophago,stomach intestine,liver),and is one of reasons for death following allogenic hematopoietic stem cell transplantation. OBJECTIVE:To explore the establishment of the mouse model of GVHD,and to provide effective animal models for experimental GVHD research. METHODS:C57BL/6(H-2b)→BALB/C(H-2d) was selected as donor and recipient of complete allotransplantation. The GVHD models were established by injection of lymphocytes and bone marrow cells of the spleen of donor mouse. Whether GVHD models were established was assessed according to clinical situation and pathological examination. RESULTS AND CONCLUSION:The recipient mice which accepted above 5×106 donor spleen cells developed acute GVHD after transplantation. However,the time when clinical situation of GVHD appeared and life span were different. Results indicated that the transfusion of 5×106 spleen cells was adequate to establish the mouse model of GVHD. The mouse model is reliable to the experimental research of GVHD.