脊髓损伤后MMP-9表达的时空分布及其意义

目的通过研究大鼠急性脊髓损伤后基质金属蛋白酶-9(MMP-9)表达的时空变化，探讨基质金属蛋白酶-9在急性脊髓损伤后的作用。方法使用改良Allen法制作大鼠急性脊髓损伤模型，检测脊髓损伤后基质金属蛋白酶-9在损伤脊髓中表达的时空分布特点。结果损伤组急性脊髓损伤后1小时，可见基质金属蛋白酶-9的表达，损伤后1～2天达到高峰，7天仍有表达。阳性细胞主要表达在缺血神经元、血管内皮细胞和嗜中性粒细胞。对照组未见基质金属蛋白酶-9表达。结论基质金属蛋白酶-9在脊髓损伤后的早期表达，提示基质金属蛋白酶-9可能参与脊髓损伤后水肿形成和炎性细胞的浸润，加重了继发性脊髓损伤。

Expression of matrix metalloproteinase-9 after acute spinal cord injury

Objective To investigate the early expression of matrix metalloproteinase-9 after actue spinal cord injury.Methods The models of spinal cord injury in rats were induced with Allen's way and the expression of matrix metalloproteinase-9 in injuried spinal cord was observed.Results The expression of matrix metalloproteinase-9 increased 6h after spinal cord injury,which reached its peak 1,2 days after spinal cord injury(SCI).The matrix metalloproteinase-9 positive cells were mainly expressed on ischemia neuron, angioendothelial cells and neutrophile.The express of matrix metalloproteinase-9 wasn't found in the sham-operated rats.Conclusion The early matrix metalloproteinase-9 may be partially involved in the secondary pathogenesis of spinal cord injury.