| Zmu-1:DHP豚鼠乳酸脱氢酶和超氧化物歧化酶同工酶的研究 |
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| 引用本文： | 刘迪文,郭汉身,胡根林,傅军. Zmu-1:DHP豚鼠乳酸脱氢酶和超氧化物歧化酶同工酶的研究[J]. 实验动物与比较医学, 1996, 0(Z1) |
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| 作者姓名： | 刘迪文 郭汉身 胡根林 傅军 |
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| 作者单位： | 浙江医科大学实验动物中心 |
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| 摘 要： | 采用聚丙烯酰胺凝胶垂直板电泳法，分析了Ｚｍｕ１：ＤＨＰ豚鼠心、肝、脾、肺、肾、骨骼肌、红细胞和血清的ＬＤＨ及ＳＯＤ同工酶，对ＬＤＨ同工酶谱作了光密度扫描，计算出各同工酶组分的相对活力及不同组织和血清ＬＤＨ中Ａ、Ｂ亚基的含量。Ｚｍｕ１：ＤＨＰ豚鼠肝中缺乏ＤＨＰ豚鼠和其他实验动物肝中普遍存在的ＬＤＨ５组分，多数组织中ＬＤＨＢ亚基含量呈不同程度提高。Ｚｍｕ１：ＤＨＰ豚鼠各组织和血清的ＳＯＤ同工酶呈多带分布。ＳＯＤ１和ＳＯＤ２为共有酶带。
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| 关 键 词： | Zmu1：DHP豚鼠乳酸脱氢酶 超氧化物岐化酶 同工酶 电泳 |
Study on LDH and SOD Isoenzymes of Zmu - l-.DHP Guinea Pigs |
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| Liu Di -wen Guo Han -shenHu Gen - lin Fu Jun. Study on LDH and SOD Isoenzymes of Zmu - l-.DHP Guinea Pigs[J]. Laboratory Animal and Comparative Medicine, 1996, 0(Z1) |
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| Authors: | Liu Di -wen Guo Han -shenHu Gen - lin Fu Jun |
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| Abstract: | Lactate dehydrogenase and superoxide dismutase isoenzymes in tissues of the heart, liver,spleen,lung,kidney,skeletal muscle, red blood cell and serum of Zmu1:DHP guinea pig were analysed with PAGE. The results revealed that LDH 5 was absent in the liver and the contents of LDH B subunit in most tissues were increased in varying degrees, and the SOD from different tissues and serum were appeared in multiband distribution on the electrophoretic pattern. Both SOD 1,and SOD 2 were the common bands in the zymograms for all the sampled tissues. |
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| Keywords: | Zmu1: DHP guinea pig LDH SOD Isoenzyme Electrophoresis |
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