脂蛋白（a）在主动脉粥样硬化病变中的定位与定量研究

脂蛋白（ａ）［Ｌｉｐｏｐｒｏｔｅｉｎ（ａ），Ｌｐ（ａ）］是动脉粥样化中的独立危险因子，我们采用免疫组织化学技术、免疫电镜技术、酶联免疫吸附法及图像分析技术，研究了正常及不同程度动脉粥样硬化病变的尸检主动脉中Ｌｐ（ａ）分布的量及形式。结果显示：动脉粥样硬化血管壁Ｌｐ（ａ）含量显著增高，各不同病变区域Ｌｐ（ａ）有其独特的分布规律，Ｌｐ（ａ）主要位于细胞外基质中，只在少数泡沫细胞内才发现有Ｌｐ（ａ）。Ａ

Detection and quantification of lipoprotein(a)in atherosclerotic lesions of human aorta

ipoprotein(a)Lp(a)]represents an independent risk factor for atherosclerotic disease. In the present study, the extent to which Lp(a) accumulated in normal and various degrees of atheromatous le-sions and the distribution pattern of Lp (a)in these lesions have been studied in thoracic aorta from the autopsy tissues by means of immunohistochemistry,immunoelectronic microscopy,enzyme-linked im-munosorbent assay and computer-assisted image processing. Lp(a)was found to be localized preferen-tially in lesion areas and had its own distribution pattern in each kind of lesion. Lp(a) associated primar-ily with extracellular matrix and was detected in only a small number of foamy cells,Apolipoprotein B was also detected,whose staining pattern was always found to be congruent with that of Lp (a). This suggests that the immunoreactivity of apoB is related to the presence of Lp(a)at least in part, since Lp(a)possesses apoB in addition to apo(a).It was concluded that both low density lipoprotein and lipopro-tein(a) play an important role in the pathogenesis of atherosclerosis.