自发性高血压大鼠脑细小动脉神经肽Y分布的研究

为了解血管周围神经肽类分布与高血压时细小动脉构型重建之关系，我们采用自发性高血压大鼠（ＳＨＲ）软脑膜铺片技术，展示了血管网的各级分支，对血管系统进行了全面、立体的观察。并用免疫组织化学、图像分析仪对血管网上神经肽Ｙ（ＮＰＹ）分布进行定性定量分析。结果表明，ＳＨＲ大脑中动脉及其分支，小动脉、细动脉壁上含ＮＰＹ神经纤维分布表面积密度及ＮＰＹ抗体阳性反应物积分光密度均明显高于正常血压组（ＷＫＹ），这一实验结果揭示脑细小动脉含ＮＰＹ神经分布密度增加，使血管平滑肌营养调节作用增强，与其动脉收缩兴奋性增高，动脉平滑肌细胞肥大和高血压的发生发展有着明显关系。

Study on neuropeptide Y-containing innervation of cerebral small arteries and arterioles in spontaneously hypertensive rat

o comprehand the relationship between the distribution of perivascular neuropeptides and remodi-fied structure of arterioles in hypertension,we applied the technique of sheeting from pia matter of spon-taneously hypertensive rats(SHR)to reveal branches at all levels of the vascular network so as to make an overall and stereoscopic observation of the vasculature. In addition,immunohistochemical technique and image analyser were applied to analyse qualitatively and quantitatively neuropeptide Y(NPY)-con-taining innervation in the vasculature. Findings revealed that the surface density of NPY-containing in-nervation and integral luminous density of positive reaction particle for anti-NPY in middle cerebral artery and its branches-small arteries and arterioles of SHR were significantly increased as compared with findings in Wistar Kyoto. The results reveal that an increase in density of NPY-containing innerva-tion of cerebral small arteries and arterioles intensifies action of nutritive adjustment of vascular smooth muscle and is clearly related with increase of contractive excitability of artery,hypertrophy of artery,smooth muscle cells and the development of hypertension.