缺氧内皮细胞培养液对肺动脉平滑肌细胞表型的影响

用细胞培养和形态定量分析方法观察缺氧对肺动脉平滑肌细胞表型的影响。结果显示，缺氧性内皮细胞条件培养液组肺动脉平滑肌细胞的二倍体细胞和α－ｓｍ－ａｃｔｉｎ含量均少于常氧性内皮细胞条件培养液组（Ｐ＜０．０５），尤以肌丝成份减少最明显；粗面内质网和线粒体却显著增多。而直接缺氧组与常氧组无明显差异。提示：缺氧可促使内皮细胞产生和释放某种促平滑肌细胞表型转化的物质或因子，从而改变了肺动脉管壁细胞之间的正常调控关系，导致平滑肌细胞肥大，合成细胞外基质增多。

Effects of hypoxia on the phenotype of porcine pulmonary arterial smooth muscle cells in vitro

he effects of hypoxia on phenotype modulation of pulmonary artery srnooth muscle cells(PASMC)was observed using cell culture and morphometry analysis.The results showed that the num-ber of diploid cells and alpha-sm-actin of the hypoxic endothelial cell condition medium group(HECCM)was lower and the myofibrilles were even less than that of the normal endothelial cell condi-tion medium group(NECCM ).On the contrary, the volume density of rough endothelial reticulum(RER )and mitochondria of the HECCM group were higher than that of the NECCM group.However,under direct hypoxic condition for 24 hours,the phenotype of cultured PASMCs remained unchanged.It suggests that the HECCM can induce phenotype mod ulation of PASMCs may be mediated by the pulmonary artery endothelial cells(PAECs)which can secrete some cytokines affecting the PASMCs.