尖锐湿疣组织中人乳头状瘤病毒的检测

用免疫组织化学、ＤＮＡ原位杂交和聚合酶链反应技术，检测人生殖器尖锐湿疣和女阴假性湿疣组织中人乳头状瘤病毒衣壳抗原（ＨＰＶ－Ａｇ）和病毒核酸序列（ＨＰＶ－ＤＮＡ），并观察了ＨＰＶ在尖锐湿疣组织中的分布特点与病变组织学改变的关系。结果显示尖锐湿疣中ＨＰＶ－Ａｇ阳性率为７１．４％（３５／４９）；原位杂交ＨＰＶ６／１１ＤＮＡ阳性率为９６．５％（２８／２９）；ＰＣＲ扩增后尖锐湿疣ＨＰＶ６／１１／１６／１８ＤＮＡ阳性率为１００％（５３／５３）；假性湿疣ＨＰＶ６／１１／１６／１８ＤＮＡ阳性率为２１．４％（３／１４）。观察ＨＰＶ－Ａｇ和ＨＰＶ－ＤＮＡ分布，表明ＨＰＶ增殖性感染和尖锐湿疣特异的病理改变密切相关。

Detettion of human papillomavtrus in condyloma acuminata tissues

e used immunohistochemical technique, in situ hybridization and polymerase chain reaction to de-tect HPV capsid antigen(HPV-Ag)and HPV-DNA sequences in genital condyloma and pseudocondy-loma of vulvae tissues.We also observed the relation between HPV distribution in condylomas and their histological changes. Results : 71.4%of condylomas were positive for HPV-Ag with IMHC ,96.5%of condylomas were positive for HPV 6/11 DNA with ISH , and 100%of condylomas were positive for HPV6/11/16/18DNA with PCR. Only 21.4%of pseudOcondylomas of vulvae were positive for HPV6/11/16/18 DNA with PCR. By observing the distribution of labelled HPV-Ag and HPV-DNA,we found a close relation between HPV proliferative infection and the specific changes of condylomas