女性下生殖道解脲脲原体的基因分型与致病性研究

目的运用反相斑点杂交技术进行解脲脲原体(UU)基因分型,揭示正常携带状态及感染状态下解脲脲原体的基因型,并建立一个能用于临床标本直接分型的简单、实用、可靠性好的解脲脲原体基因分型方法。方法对有阴道炎症状和体征的病例组和正常体检人群进行病史询问、临床检查,先取阴道分泌物,采用FQ-PCR做解脲脲原体检测。设计分型引物,对FQ-PCR阳性标本用反相斑点杂交进行解脲脲原体基因型鉴定。结果病例组与对照组解脲脲原体基因型的检出率差异无显著性,总检出率为89.1%。对照组中解脲脲原体以parvum群(微小脲原体)为主(79.3%),其检出率较女性下生殖道感染患者高(P=0.018),而且以其中的1,3,6基因型的单型别为主(82.6%)。病例组解脲脲原体两群感染(16.2%)较对照组高(6.9%),差异有显著性(P=0.033),其中以T960群与Parvum群1型的多型别感染较高(P=0.009);在多型别感染中1型的检出率病例组较对照组高(P=0.026),而单型别感染中1型的检出率在对照组高(P=0.000)。结论parvum群,尤其是1,3,6的单型别在正常人群携带的可能性较大。而女性下生殖道感染患者解脲脲原体的两群感染较正常体检人群高,尤以T960群与parvum群中1型的多型别感染较正常体检人群高,提示T960群有可能和1型起协同作用或独自导致疾病。

Genotyping and Pathogenicity of Ureaplasma Urealyticum in Female Genital Tracts

Objective To determine the distribution of different Ureaplasma urealyticum genotypes among physical check-up women and the gential tract infection patients;Another aim of this study was to establish Reverse dob blot hybridization for rapid direction of genotypes of Ureaplasma urealyticum,to assess its value in clinical applications.Methods Physical examinations were undertaken for all patients who had vulvar itching and vaginal discharge manifold and for women who with medical examination.We got the weight of secretion by differential subtract method then to detect for U.urealyticum by fluorescent quantitative PCR(FQ-PCR).The specimen from suspected lesions were tested for HSV and HPV and the serological tests were carried out for syphilis.Primers for typing were designed and to detect genotype of Ureaplasma urealyticum by Reverse dot blot hybridization(RDB).Results The detection rate of genotype of Ureaplasma urealyticum was no significant difference between the above of two groups,Total detection rate of two groups was 89.1%.Infection with only one genotype(genotype 1,3 or 6) of U.parvum is frequently found in control group;Two-biovar infection and T960 biovar mixed with genotype 1 were more prevalent that in patients in case group than in control group women(P=0.033 and P=0.009,respectively).Conclusion People Infected with only one genotype(genotype 1,3 or 6) of U.parvum may be normal carriers.Two-biovar infection and T960 biovar mixed with genotype 1 were more prevalent in patients with the genital infection than that in normal women taking medical examination.T960 biovars mixed with genotype 1 may be more frequently associated with the genitial tract infection.The RDB method described here is relatively simple,rapid for biotyping between U.parvum and U.urealyticum.It may be used as a tool in rapid diagnosing for genotype of Ureaplasma urealyticum.