不同类型子宫内膜腺上皮细胞中bcl-2和bax基因表达的研究

目的:探讨bcl-2、bax在不同类型子宫内膜腺上皮细胞中的表达。方法:采用免疫组化检测子宫内膜腺上皮细胞中二指标的表达。结果:从单纯性、复杂性到不典型增生,bcl-2和bax的表达评分均有逐渐增多的趋势,不典型增生与前二者相比差异均有统计学意义,P<0·01。在内膜样腺癌中,随着分化程度的降低、分期的增加,bcl-2表达评分下降。随着分化程度的降低bax的表达逐渐降低;随着浸润深度的增加bax表达逐渐增加。在增生期和分泌期bcl-2和bax的表达无负相关,但在内膜样腺癌组织中bcl-2表达与bax呈正相关。结论:bcl-2和bax参与了癌前病变的发生,bcl-2、bax表达水平在一定程度上反映了内膜样腺癌的生物学行为。bcl-2和bax在增生期和分泌期内膜变化中的相互作用关系不大,但二者共同作用参与了内膜样腺癌的发生与发展。

Research on bcl-2 and bax gene expression in gland epithelial cells of various endometriums

OBJECTIVE: To analyze the expression of bcl-2 and bax in the gland epithelial cells of various endometriums.METHODS:The immunohistochemical method was used to detect the bcl-2 and bax expression in various endometriums.RESULTS: bcl-2 and bax became increasing from simple hyperplasia (SH), complex hyperplasia(CH) to atypical hyperplasia (AH). bcl-2 and bax were higher in AH than in SH and CH, and the differences were significant,P<0.01. In the endometrioid adenocarcinoma (EAC), bcl-2 expression were associated with the differentiation grades (DG) and surgical pathological stages.As for bax, poorer of DG, shorter of bax expression; more deeper of myometrial invasion, more of bax expression. bcl-2 expression had no negative correlation with bax, but there were positive correlations between expression of bcl-2 and bax in EAC. CONCLUSIONS:bcl-2 and bax may participate in the origin of precancerous lesions to a certain extent, bcl-2 and bax expression may reflect the DG and biological behavior. They could be important parameters for the prognosis of patients, and bcl-2 and bax may jointly put important role in the development of EAC.