粪便乳杆菌 FZB1菌株的益生特性研究?

目的：研究粪便乳杆菌 FZB1菌株的益生特性。方法采用 API 50 CH 乳酸菌鉴定试剂条测定碳源利用能力。通过生长实验测定粪便乳杆菌 FZB1菌株的耐酸性和对不同胆盐的耐受性。用荧光标记法，以人结肠癌细胞 HT-29作为体外模型，研究粪便乳杆菌 FZB1菌株的黏附性能及对病原菌大肠杆菌 E57黏附抑制性能。结果试验结果显示，粪便乳杆菌 FZB1菌株可利用多种碳源和乳糖，耐受 pH 3.0的酸度，可在0.4%牛磺石胆酸钠、0.08%胆酸钠、0.15%禽胆盐、0.15%脱氧胆酸钠、0.15%牛磺鹅脱氧胆酸钠和0.06%牛胆盐环境中生长。粪便乳杆菌 FZB1菌株对 HT-29细胞的黏附率可达79.9%~86.9%，能通过竞争、排斥、置换作用阻止大肠杆菌 E57的黏附，黏附率分别为82.1%~86.3%、79.6%~83.2%和79.5%~82.5%。结论粪便乳杆菌 FZB1菌株具有降解乳糖、耐酸、耐胆盐和在肠道定植黏附的能力，适合在鸡、鸭、鹅等家禽肠道中发挥益生作用。

A Study on the Probiotic Properties of FZB1 Strains of Lactobacillus Faecis

Objective To analyze the probiotic properties of FZB1 stains of Lactobacillus faecis .Methods The API 50 CH system was used to determine the utilization of carbon sources.The growth experiment was adopted to detect the FZB1 stains′acid tolerance and their tolerance to different bile salts.The fluorescence labeling method was used to investigate the adhesive properties and the inhibitory effect on the adherence of pathogen with the HT-29 cell line as an in vitro model.Results The results showed that the FZB1 stains of Lactobacillus faecis were capable of using various carbon sources and lactose,being resistant to pH 3.0,and growing in 0.4%taurolithocholic acid sodium,0.08% sodium cholate,0.1 5% poultry bile salt,0.1 5% sodium deoxycholate,0.1 5%taurocholate chenodeoxycholic acid sodium and 0.06% bile salt respectively.The results of adhesion showed that the rate of the FZB1 strains′ adhesion to the HT-29 cell line was 79.9%-86.9%,and the FZB1 strains inhibited the adhesion of Escherichia coli E5 7 by means of competition,exclusion and replacement with the adhesion rates of 82.1%-86.3%,79.6%-83.2% and 79.5%-82.5% respectively.Conclusion The FZB1 strains of Lactobacillus faecis is capable of lactose degradation,acid tolerance,bile salt tolerance and adhesion to intestinal colonization,and their probiotic properties have a beneficial effect on the intestines of the poultry such as chicken,ducks,geese,etc.