人类免疫缺陷病毒感染抗体检测窗口期血清的检出

目的　搜索高危人群中HIV抗体“窗口期”样品。方法　使用HIV抗体检测、HIV 1 p2 4抗原检测和病毒载量检测等多种方法对 1 580份高危人群血清和血浆进行筛查。结果　发现 1份样品抗体检测为阴性 ,p2 4抗原检测为阳性 ,病毒载量HIV 1RNA含量极高 ,拷贝数为每毫升 41万 (RT PCR法 )和 76万 (NASB法 )。结论　该份样品HIV抗体水平极低 ,用国内目前使用的进口和国产HIV抗体检测试剂均不能检出 ,确定其为 1份处于HIV感染抗体窗口期的样品

A Sample in the HIV-1 Antibody Window Phase Deteated by HIV-1 RNA and p24 Antigen Testing

Objective To screen 1580 serum samples of high risk population to find if there are any samples in window phase.Methods Kits for detection of HIV 1/2 antibodies,HIV 1 p24 antigen and HIV-1 viral load were used for screening.Results One sample was found positive for p24 antigen and negative for HIV antibody.The viral load of the sample was 414000 HIV RNA copies per ml by Roche RT PCR method and 760000 HIV RNA copies per ml by NASBA method.Conclusion Based on the above data,this sample was in the window period of HIV infection.However,the concentration of antiHIV antibodies in this sample was too low to be detected by domestic or imported kits for detection of HIV1/2 antibodies.