凋亡相关蛋白Survivin及Caspase-3在膀胱移行细胞癌中表达及临床意义

目的：探讨凋亡相关蛋白Survivin及Caspase-3在膀胱移形细胞癌表达及其临床意义。方法：应用SP免疫组织化学法检测45例膀胱移行细胞癌及10例正常膀胱黏膜组织石蜡切片中Survivin和Caspase-3表达的情况，结合临床资料进行分析。结果：Survivin在膀胱移形细胞癌标本中的表达阳性率为68．9%（31/15）．而正常对照组中无一例呈阳性表达；Caspase-3在膀胱移行细胞癌标本中的表达阳性率为37．8%（17/45）．与对照组阳性率90％（9／10）相比差异有统计学意义（P〈O．05）。Survivin的表达与膀胱移行细胞癌的组织学分级、初发和复发显著相关（P〈O．05），但与临床病理分期、肿瘤数日无关；Caspase-3的表达与膀胱移行细胞确的初发复发相关，但与组织学分级、肿瘤数目、临床分期均无关。相关性分析表明，膀胱移行细胞癌中Survivin的表达与Caspase-3表达呈负相关。结论：Survivin在膀胱癌组织中选择性表达与膀胱移行细胞癌的分化程度密切相关．Caspase-3蛋白在膀胱移行细胞癌中表达下降，Survivin及Caspase-3蛋白对于判断膀胱移行细胞确预后有重要临床指导意义。

Expression of Survivin and Caspase-3 in transitional cell carcinoma of bladder and their significance

Objective:To investigate the expression of Survivin and Caspase 3 in transitional cell carcinoma(TCC)of bladder and their clinical significance.Methods:The expression of Survivin and Caspase-3 was detected by streptavidin-peroxidase immunohistochemical method in the paraffin embedded sections of 45 TCC patients with the clinical data analyzed.Normal bladder tissues from 10 health people were used as control group.Results:The positive rate of the expression of Survivin in TCC was 68.9%(31/45),while none of control group expressed Survivin.The positive rate of the expression of Caspase 3 in TCC was 37.8%(17/45).The expression of Survivin was related to the histological grades and recurrence but no significant difference was detected in different pathological stages,while the expression of Caspase-3 was significantly suppressed in recurrent cases.There was a negative relation between the expression of Survivin and Caspase 3 in TCC.Conclusions:The expression of Survivin in TCC was associated with the histological grades.The expression of Caspase 3 was suppressed in TCC.High expression of surviving and low expression of Caspase-3 indicate poor prognosis of BTCC.