氨基胍对创伤性休克大鼠血浆一氧化氮及内皮素变化的影响

目的　探讨一氧化氮合酶抑制剂氨基胍对创伤性休克过程中血浆一氧化氮、内皮素及组织氧分压的影响。方法　采用股骨创伤法建立创伤性休克大鼠模型 ,随机分为对照组与处理组。观察两组大鼠创伤前后及复苏后 1、 3、 5、 12h血浆一氧化氮、内皮素及骨骼肌、肝脏、小肠的组织氧分压的动态变化 ,监测血液动力学变化并记录存活时间。结果　两组大鼠休克末及复苏后各时间点血浆一氧化氮、内皮素浓度及组织氧分压较伤前差异有显著性意义 (P <0 0 5 ) ;同对照组相比 ,处理组休克后期一氧化氮及内皮素浓度明显降低 ,差异有显著性意义 (P <0 0 5 ) ,而休克后期肝脏、小肠氧分压明显高于对照组 ,差异有显著性意义 (P <0 0 5 ) ,处理组复苏后血压明显高于对照组 ,其 12、 2 4存活率也明显高于对照组 ,差异有显著性意义 (P <0 0 5 )。结论　氨基胍可降低血浆一氧化氮、内皮素浓度 ,提高血压 ,改善内脏器官的组织氧分压 ,从而改善创伤性休克大鼠的预后

Effect of aminoguanidine on dynamic changes of plasma nitric oxide and endothelin on traumatic shock in rats

Objective To evaluate the effect of aminoguanidine(AG),one kind of nitric oxide synthese(NOS)antagonist,on the plasma concentration of nitric oxide(NO),endothelin(ET)and oxygen partial pressure in tissues during the pathogenesis of traumatic shock in rats.Methods Rat model of traumatic shock was established in Sprague-Daulay rats,which were randomly divided into two groups:control group and treatment group.The plasma concentration of NO,ET and oxygen partial pressure in the tissues from the skeletal muscles,liver and small intestine were measured before and after shock and 1,3,5,and 12h after resuscitation.Meanwhile,the hemodynamics of the rat and their survival rates at 12 and 24h were recorded.Results The changes of plasma NO,ET levels and oxygen partial pressure in tissues of both groups were statistically significant after traumatic shock(P<0.05).Plasma NO and ET concentration in the late stage of traumatic shock were significantly lower in the treatment group than in the control group(P<0.05),while oxygen partial pressure in the liver and small intestine in the late stage of shock were significantly higher in the treatment group(P<0.05).The blood pressure after resuscitation and the survival rates at 12 and 24h were also significantly different between the two groups(P<0.05).Conclusion AG can decrease plasma NO and ET levels,improve blood pressure and oxygen partial pressure of internal organs which can significantly improve the prognosis of rats with traumatic shock.