肝细胞凋亡及凋亡相关基因Caspase-3、Bcl-2在慢性乙型重型肝炎中的作用

目的 探讨肝细胞凋亡及凋亡相关基因Caspase-3、Bcl-2在慢性乙型重型肝炎(慢重肝)发生发展机制中的作用.方法 选择慢重肝肝组织标本68例(慢重肝组),正常肝组织标本20例(对照组).采用原位末端转移酶标记技术(terminaldeoxynucleotidyl transferase-mediated dUTP...

Role of hepatocyte apoptosis and apoptosis-related genes Caspase-3 and Bcl-2 in the pathogenesis of chronic severe hepatitis B

Objective To investigate the role of hepatocyte apoptosis and apoptosis-related genes Caspase-3 and Bcl-2 in the pathogenesis of chronic severe hepatitis B. Methods Samples of liver tissues from 68 patients with chronic severe hepatitis B and 20 healthy controls were detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay and immunohistochemical staining in order to observe the expression of Caspase-3 and Bcl-2 and hepatocyte apoptosis. Results The mean apoptotic index (AI) in chronic severe hepatitis B group was 41.6±4.9, while in the control group no positive expression of hepatocytes was detected by TUNEL assay. The difference between the two groups was statistically significant (P<0.01). Positive expression of Caspase-3 in chronic severe hepatitis B group was significantly higher than that in the control group (P<0.05), while that of Bcl-2 in chronic severe hepatitis B group was significantly lower than that in the control group (P<0.05). According to Spearman analysis, there was a positive correlation between AI and the expression of Caspase-3 (r=0.592, P<0.01), and a negative correlation between AI and the expression of Bcl-2 (r=-0.461, P<0.05) in the chronic severe hepatitis B group. Conclusions Hepatocyte apoptosis may play a significant role in the pathogenesis of chronic severe hepatitis B. Caspase-3 and Bcl-2 participate in the hepatocyte apop- tosis of chronic severe hepatitis B.