maFGF对慢性衰老大鼠肝组织ATP酶、SOD活力及丙二醛和羟自由基水平的影响

目的探讨改构型酸性成纤维细胞生长因子(maFGF)对衰老大鼠肝组织中ATP酶、超氧化物歧化酶(SOD)活力及丙二醛(MDA)、羟自由基(OH.)水平的影响。方法采用皮下注射D-半乳糖建立衰老大鼠模型,将其随机分为衰老模型组、生理盐水对照组和maFGF治疗组。另10只不给任何药物,正常饲养,作为正常对照组。测定各组肝组织中Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和SOD活力以及MDA、OH.水平。结果衰老模型组大鼠肝组织Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和SOD活力均显著降低,MDA和OH.基含量均升高(P<0.05);maFGF治疗组大鼠肝组织Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和SOD活力均显著升高,MDA和OH.含量均降低(P<0.05)。结论 maFGF通过降低自由基,提高ATP酶活力和抗氧化能力而对衰老大鼠有保护作用。

Effects of maFGF on activities of ATPase and SOD,levels of malondialdehyde and hydroxyl radical in liver tissue of chronic aging rats

Objective To study the effects of modified acidic fibroblast growth factor(maFGF) on activities of ATPase and superoxide dismutase(SOD),levels of malondialdehyde(MDA) and hydroxyl radical(OH·) in liver tissue of aging rats.Methods Aging rat models were established by subcutaneous injection of D-galactose.They were randomly divided into aging model group,physiological saline control group and maFGF treatment group.Other 10 rats accepted drug-free normal feeding served as normal control group.Activities of Na+-K+-ATPase,Ca2+-Mg2+-ATPase and SOD,levels of MDA and OH· in liver tissue were measured.Results In aging model group,activities of Na+-K+-ATPase,Ca2+-Mg2+-ATPase and SOD in liver tissue of rats were markedly decreased,while levels of MDA and OH·increased(P<0.05).In maFGF treatment group,activities of Na+-K+-ATPase,Ca2+-Mg2+-ATPase and SOD in liver tissue of rats were significantly increased,while levels of MDA and OH· decreased(P<0.05).Conclusion maFGF exhibit a protective effect on aging rats via reducing free radicals,enhancing ATPase activities and antioxidant capacity.