| 结核分枝杆菌PPE68基因真核表达质粒的构建及鉴定 |
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| 引用本文： | 靳志栋,何永林,徐蕾,佘茜,张丹,张壮苗,杨春.结核分枝杆菌PPE68基因真核表达质粒的构建及鉴定[J].重庆医科大学学报,2011,36(8):907-910. |
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| 作者姓名： | 靳志栋 何永林 徐蕾 佘茜 张丹 张壮苗 杨春 |
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| 作者单位： | 重庆医科大学病原生物学教研室、重庆医科大学神经科学研究中心、重庆市重点实验室,重庆,400016 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:构建结核分枝杆菌(Mycobacteriatuberculosis,Mtb)PPE68基因的真核表达质粒,观察PPE68基因在小鼠巨噬细胞株RAW264.7中的表达.方法:将MtbPPE68基因克隆亚至真核表达质粒pBudCE4.1中,构建真核表达质粒pBudCE4.1-PPE68.以真核表达质粒pBudCE4....
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| 关 键 词： | Mtb PPE68 巨噬细胞 |
Construction and identification of eukaryotic expression plasmid pBudCE4.1-PPE68 of Mycobacterium tuberculosis |
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| JIN Zhi-dong,HE Yong-lin,XU Lei,SHE Qian,ZHANG Dan,ZHANG Zhuang-miao,YANG Chun.Construction and identification of eukaryotic expression plasmid pBudCE4.1-PPE68 of Mycobacterium tuberculosis[J].Journal of Chongqing Medical University,2011,36(8):907-910. |
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| Authors: | JIN Zhi-dong HE Yong-lin XU Lei SHE Qian ZHANG Dan ZHANG Zhuang-miao YANG Chun |
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| Institution: | JIN Zhi-dong,HE Yong-lin,XU Lei,SHE Qian,ZHANG Dan,ZHANG Zhuang-miao,YANG Chun(Department of Pathobiology,Institute of Neuroscience Chongqing Medical Univercity,Chongqing Key Laboratory of Neurobiology) |
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| Abstract: | Objective:To construct and identify eukaryotic expression plasmid pBudCE4.1-PPE68 and observe its expression in murine macrophage RAW264.7 cells.Methods:MTB PPE68 gene was subcloned into pBudCE4.1 to construct recombinant plasmid pBudCE4.1-PPE68.The recombinant plasmid was transiently transfected into RAW264.7 cells,and the expression of PPE68 gene was detected by RT-PCR and Western blot.Results:The accurate eukaryotic recombinant plasmid pBudCE4.1-PPE68 was identified by restrict endonuclease.The PPE68 gen... |
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| Keywords: | mycobacterium tuberculosis PPE68 macrophage |
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