慢性阻塞性肺疾病急性加重期患者血浆SP-D水平测定及临床意义

目的检测慢性阻塞性肺疾病(COPD)急性加重期及稳定期患者血浆肺表面活性物质相关蛋白D(SP-D)含量并探讨其临床意义。方法选择COPD急性加重期(AECOPD)患者住院治疗时及治疗后获得缓解的患者69例,采用双抗体夹心酶标免疫分析法检测血浆SP-D浓度,同时进行血常规检测。检测42例健康体检者作为对照。结果 AECOPD及稳定期COPD患者血浆SP-D平均浓度分别为(183±86)、(126±72)ng/mL,对照组为(96±47)ng/mL,AECOPD与稳定期、对照组比较差异有统计学意义(P<0.01),AECOPD患者血浆SP-D阳性率为85.50%,与WBC、中性粒细胞的阳性率比较差异有统计学意义(P<0.01)。结论血浆SP-D可作为AECOPD诊断的潜在生物学指标。

Detection and its clinical significance of serum SP-D level in patients with acute exacerbation of chronic obstructive pulmonary disease

Objective To detect the content of serum pulmonary surfatcant-associated protein D(SP-D) in patients with chronic obstructive pulmonary disease(COPD) during acute exacerbation and in stable phase and discuss its clinical significance.Methods 69 patients with acute exacerbation of COPD(AECOPD) which achieved remission during and after hospital treatment were selected.Double-antibody sandwich enzyme linked immunosorbent assay was employed to measure the concentration of serum SP-D,and the complete blood count test was performed simultaneously.42 cases of healthy people accepted physical examine served as control.Results Average concentration of serum SP-D in patients with AECOPD during acute exacerbation and in stable phase were(183±86),(126±72) ng/mL,respectively,while that in control group(96±47) ng/mL].There were statistically significant differences between patients with AECOPD and patients in stable phase or in control group(P<0.01).The positive rate of serum SP-DD in patients with AECOPD were 85.50% which demonstrated statistical difference when compared with those of WBC and neutrophils(P<0.01).Conclusion Serum SP-D may be a potential biological indicator for AECOPD diagnosis.