| 人Api6融合蛋白原核表达系统的构建 |
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| 引用本文： | 方严,练雪梅. 人Api6融合蛋白原核表达系统的构建[J]. 重庆医科大学学报, 2011, 36(9): 1069-1072 |
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| 作者姓名： | 方严 练雪梅 |
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| 作者单位： | 1. 重庆医科大学公共卫生学院实验室
2. 重庆医科大学公共卫生学院营养与食品卫生学教研室,重庆,400016 |
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| 摘 要： | 目的:本研究拟采用蛋白原核表达的方法,诱导表达人Api6(Apoptosis inhibitor 6)融合蛋白,为进一步研究Api6在凋亡抑制、脂质代谢和肿瘤发生中的作用奠定基础.方法:利用PCR技术扩增人Api6基因,将其克隆至原核表达载体ppSUMO,构建重组质粒ppSUMO-Api6;重组质粒转化原核表达宿主菌E...
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| 关 键 词： | 凋亡抑制因子6 原核表达 融合蛋白 |
The construction of prokaryotic expression system for human Api6 fusion protein |
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| FANG Yan,LIA N Xue-mei. The construction of prokaryotic expression system for human Api6 fusion protein[J]. Journal of Chongqing Medical University, 2011, 36(9): 1069-1072 |
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| Authors: | FANG Yan LIA N Xue-mei |
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| Affiliation: | FANG Yan1,LIAN Xue-mei2(1.Centre for Lipid Research,Key Laboratory of Molecular Biology on Infectious Disease,Ministry of Education,Chongqing Medical University,2.Department of Nutrition and Food Hygiene,School of Public Health,Chongqing Medical University) |
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| Abstract: | Objective:To further study the pathophysiological function of human Api6 fusion protein expression using a prokaryotic expression system.Methods:Api6 cDNA was inserted into ppSUMO vector to form recombinant plasmid ppSUMO-Api6;recombinant plasmid ppSUMO-Api6 was transformed into prokaryotic expression host E.coli Rosetta;prokaryotic expression method was optimized to induce expression of recombinant protein Api6 in different conditions of E.coli density,temperature,IPTG concentration and induction time;reco... |
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| Keywords: | Api6 prokaryotic expression fusion protein |
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