冠心病患者肺炎衣原体包涵体的检测和意义

目的:探讨冠心病(CHD)患者血液肺炎衣原体(Cpn)包涵体的检测和临床意义。方法:收集2006年CHD患者150例和同期体检正常者55例的血液标本,采用-85℃冰冻保存的外周抗凝血液,低渗溶血制备淋巴细胞悬液,用直接免疫荧光法(DIF)检测特异性Cpn包涵体。结果:冠心病组150例,包涵体阳性20例,阳性率为13.3%;健康对照组55例,包涵体阳性1例,阳性率为1.8%,差异有统计学意义(P<0.001),相对危险度为8.3,95%的可信区间为1.09～63.46。结论:CHD患者Cpn包涵体阳性率高于健康者,说明CHD患者存在Cpn感染状况。

Detection of Chlamydia pneumoniae inclusion body in patients with coronary heart disease

Objective:To explore the value of Chlamydia pneumonia(Cpn)inclusion body detection in peripheral blood mononuclear cell(PBMC)of patients with coronary heart disease(CHD).Methods:The anticoagulant peripheral blood of CHD patients were preserved at-85 ℃.and lymphocyte suspension were prepared with hypoosmotic hemolysis.Cpn inclusion body was detected by direct immunofluorescence(DIF).Results:Cpn inclusion bodies was detected in 20(13.3%)of 150 patients with CHD and in 1(1.8%)of 55 in healthy control group.The positive rate of inclusion bodies in CHD group was significant higher than those in control group(P<0.001).Conclusion:Patients with CHD have higher showing evidence for the presence of Cpn inclusion bodies and was infecting