| 肝细胞癌病人中p53基因突变状况探讨 |
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| 引用本文： | 康光明,方志正,Martin Volkmann. 肝细胞癌病人中p53基因突变状况探讨[J]. 中华实验和临床病毒学杂志, 2001, 15(2): 163-165 |
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| 作者姓名： | 康光明 方志正 Martin Volkmann |
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| 作者单位： | 1. 卫生部武汉生物制品研究所
2. Zentrallar of Medicine Klinik PoliklinikHedelberg University |
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| 摘 要： | 目的 了解肿瘤抑制基因p53在肝细胞癌中的突变情况，探讨乙型肝炎病毒（HBV）感染与p53基因突变之间的关系。方法 提取50例有乙型肝炎病毒感染史肝癌患者手术样本中的DNA，用聚合酶链式反应（PCR）扩增5－9外显子，作单链构象多态性（SSCP）分析。结果 p53基因突变率超过26％，突变主要分布于5－8外显子，5、6、7、8外显子分别有3、3、4、3例，另仍4个可疑突变。结论 p53基因突变可能是肝细胞癌的病因之一，而乙型肝炎病毒感染在中国肝癌患者p53基因突变中可能起到比较重要的作用。
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| 关 键 词： | p53基因 乙型肝炎病毒 聚合酶链反应 单链DNA DNA突变分析 肝细胞癌 |
| 修稿时间： | 2000-04-24 |
Study on p53 gene mutation in hepatocellular carcinoma patients of China |
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| KANG Guangming,FANG Zhizheng,Martin Volkmann. Study on p53 gene mutation in hepatocellular carcinoma patients of China[J]. Chinese journal of experimental and clinical virology, 2001, 15(2): 163-165 |
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| Authors: | KANG Guangming FANG Zhizheng Martin Volkmann |
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| Affiliation: | Wuhan Institute of Biological Products, Wuhan 430060, China. |
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| Abstract: | OBJECTIVE: To study p53 gene mutation in human hepatocellular carcinoma (HCC) samples and the relationship between p53 gene mutation and hepatitis B virus (HBV) infection. METHODS: DNA samples were prepared from 50 specimens of HCC patients that had been infected with HBV. Exons 5-9 were amplified with polymerase chain reaction (PCR), and then detected by single strand conformation polymorphism (SSCP). RESULTS: Over 26% (13/50) of p53 DNA samples were found to be mutated, mainly in exons 5-8 relating to 3,3,4,3 cases respectively and along with 4 suspicious samples. CONCLUSION: p53 gene mutation might be one of the causes of HCC, and HBV infection may be associated with such mutation in China. |
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| Keywords: | p53 gene Hepatitis B virus polymerase chain reaction DNA single strand DNA mutational analysis |
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