体外培养人胚晶状体上皮细胞生长特性的研究

目的观察体外培养人胚晶状体上皮细胞的生物学特性。方法组织块贴壁法培养人胚晶状体上皮细胞,通过倒置相差显微镜、透射电子显微镜,采用免疫细胞化学法分析人胚晶状体上皮细胞的生长特性。结果体外培养的原代人胚晶状体上皮细胞在组织块贴壁48～72 h后从组织块边缘长出,具有上皮细胞的形态特点,第10天达到融合。第二代人胚晶状体上皮生长曲线近＂S＂形,经过1～2 d的潜伏期后进入对数生长期,需10 d左右达到融合,细胞形态呈六角形或椭圆形、超微结构保持正常,免疫细胞化学SABC法染色结果显示细胞浆内α-晶状体蛋白染色阳性。结论人胚晶状体上皮细胞具有增殖能力,第二代人胚晶状体上皮细胞是进行白内障相关研究的合适实验对象。

Growth features of human embryonic lens epithelial cells cultured in vitro

Objective To investigate the biological characteristics of human embryonic lens epithelial cells cultured in vitro.Methods Human embryonic lens epithelial cells were cultured by the explanting culture methods.An inverted phase contrast microscope,electron microscope and immunocytochemistry were used to study the growth specialties of the human embryonic lens epithelial cells. Results Human embryonic lens epithelial cells of primary culture in vitro grew out of the edge of tissue pieces after incubation for 48-72 hours.The primary cultured cells maintained typical morphology of the epithelial cells and reached a confluence in 10 days.Growth curves of cultured human embryonic lens epithelial cells in passage 2 showed an S-shape.After a 1-2 day latent period,the cultured cells logarithmically grew and reached a confluence in 10 days.The shape of human embryonic lens epithelial cells in passage 2 was hexagon or oval with normal ultra-structure.Positive staining of α-crystalline was shown in passage 2 cultured human embryonic lens epithelial cells by immunocytochemistry.Conclusion Human embryonic lens epithelial cells have proliferative capacity and are suitable for related study of cataracts.