人重组胰岛素样生长因子1对慢性阻塞性肺疾病大鼠膈肌细胞凋亡的保护作用及机制探讨

目的 探讨人重组胰岛素样生长因子1(rhIGF-1)对慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)大鼠膈肌细胞凋亡的保护作用及其可能机制.方法 雄性Wistar大鼠45只,随机分为对照组(A组),COPD模型组(B组),COPD模型+rhIGF-1干预组(C组),每组15只.第1天、第14天和第28天各组处死5只,取完整膈肌称重后用原位末端标记法测定各组膈肌细胞凋亡率,免疫组织化学法测定膈肌细胞Fas蛋白表达情况,RT-PCR检测各组膈肌细胞Fas基因表达水平.结果 第28天后B、C组膈肌质量均小于A组,C组大于B组(P＜0.05).同一时间点B、C组膈肌细胞凋亡率.Fas蛋白阳性细咆率及Fas基因表达水平均大于A组,C组小于B组(P＜0.05).结论 Fas/FasL介导的凋亡途径参与了膈肌凋亡的发生,rhIGF-1可能通过干预Fas/FasL途径减少了膈肌凋亡,对COPD大鼠的膈肌凋亡产生保护作用.

Preventive effect of rhIGF-1 on diaphgramatic muscle cell apoptosis in rats with chronic obstructive pulmonary disease and mechanism

Objective To investigate the prevetive effect and mechanism of rhIGF-1 on apoptosis of diaphgramatic muscle cell in rats with chronic obstructive pulmonary disease(COPD).Methods Forty-five male Wistar rats were randomly divided into control group(group A),COPD model group(group B),COPD model+rhIGF-1 intervention group(group C),15 in each group.Five rats in each group were killed respectively at a time on the first day,14th,28th day.Take complete diaphragms to detect rate of apoptosis with TUNEL method,detect Fas protein expression with immunohistochemistry and detect Fas gene expression with RT-PCR after weighing.Results Twenty-eight days later,the mass of diaphragms of group B and C was lighter than that of group A(P＜0.05),group C heavier than group B(P＜0.05).At the same time point,rate of apoptosis,Fas protein expression and Fas gene expression of group B and C were higher than those of group A,group C was lower than group B(P＜0.05).Conclusions Fas/FasL mediated apoptosis way involves in the diaphragm apoptosis,rhIGF-1 may interfere with Fas/FasL way to reduce the apoptosis of the diaphragm and may have a protective effect of diaphragm apoptosis.