荧光原位杂交技术在检测胎儿染色体数目异常中的应用 |
| |
引用本文: | 赵一梅,郑梅玲,化爱玲,张月莲,张桂林,贺江梅. 荧光原位杂交技术在检测胎儿染色体数目异常中的应用[J]. 中国优生与遗传杂志, 2009, 17(7): 36-37 |
| |
作者姓名: | 赵一梅 郑梅玲 化爱玲 张月莲 张桂林 贺江梅 |
| |
作者单位: | 山西医科大学第一医院遗传室,太原,030001 |
| |
摘 要: | 目的探讨荧光原位杂交(fluorescence in situ hybridization,FISH)技术在检测胎儿染色体数目异常中的临床应用价值。方法对50例孕16~22周妊娠妇女羊水间期细胞进行FISH(13、21、18、X、Y)快速产前诊断,以常规羊水细胞培养染色体核型分析作为FISH检测结果对照。结果被检50例羊水间期细胞均获得诊断结果,其中48例为正常胎儿,两例为异常胎儿(1例为48,XXY+21,另1例为47,XXX)。FISH检测与常规染色体核型分析结果一致。结论FISH检测胎儿染色体数目异常具有快速、简便等优点,结果准确可靠,有较大临床应用价值。
|
关 键 词: | 荧光原位杂交 产前诊断 羊水细胞 |
To evaluate the feasibility of using FISH for the detection of fetal chromosomal abnormalities |
| |
Affiliation: | ZHAO Yi - mei, ZHENG Mei -ling, et al. ( Depat. of Genetics of the First Hospital of Shanxi Medical University, 030001) |
| |
Abstract: | Objective: To evaluate the feasibility of using fluorescence in situ hybridization (FISH) for the detection of fetal chromosomal abnormalities. Methods: Amniotic fluid samples were taken from 50 women at 16 -22 weeks of pregnancy; FISH was performed for rapid prenatal diagnosis (aneuploidies of five chromosomes 13, 21, 18, X and Y). Then the karyotypes from standard cytogenetic analysis was compared to the FISH results. Results: Each of the 50 uncultured amniotic fluid samples tested with FISH have results, forty - eight samples were normal, two samples were abnormal ( one sample was 48, XXY + 21, the other was 47, XXX). Conclusion: FISH analysis of fetal chromosomal abnormalities is a rapid, easy, accurate and very sensitive method. It could be used in clinical diagnosis. |
| |
Keywords: | Fluorescence in situ hybridization Prenatal diagnosis Amniotic fluid cells |
本文献已被 维普 万方数据 等数据库收录! |
|