反相高效液相色谱法测定人血中环孢素A质量浓度

目的建立监测人全血中环孢素A质量浓度的反相高效液相色谱（RP-HPLC）法。方法采用反相C18色谱柱，流动相为乙腈-甲醇-水（160：30-15），柱温70℃，流速0、8mL／min，检测波长210nm。结果环孢素A质量浓度在15—1600ng／mL范围内与内标的峰高比有良好的线性关系（r=0．9993），最低检测限为5ng／mL，高、中、低3种质量浓度平均回收率为96．02％～98．31％，日内精密度RSD为1．24％～3．37％（n=5），日间精密度RSD为3．45％～4．27％（n=5）。结论该法简单、准确、经济，可用于环孢素A的血药浓度监测。

Detecting Concentration of Cyclosporine A in Human Whole Blood by RP- HPLC

Objective To establish a method to detect the concentration of cyclosporine A in human whole blood, Methods C18 column was adopted and the temperature was set at 70℃.The mobile phase was a mixture of acetonitrile, methanol and water （160：30：15） and the flow rate was 0. 8mL/min. The UV detector was set at 210nm. Results The calibration curve was linear in the concentration range from 15 ng/mL to 1 600 ng/mL in blood （r=0, 999 3）. Within the range,the recovery rate of low,moderate and high concentration was 96.02%-98.31%.The lowest limit of quantitation was 5 ng/mL. The intraday precision was 1.24%-3.37% （n=5）.The interday precision was 3.45% -4. 27% （n = 5）. Conclusion This method is easy, convenient and precise to detect the concentration of cyclosporin A in blood.