高渗氯化钠羟乙基淀粉40注射液对大鼠全身高温模型血脑屏障通透性的影响

目的 观察高渗氯化钠羟乙基淀粉40注射液对大鼠全身高温模型血脑屏障(blood brain barrier.BBB)通透性的影响并探讨其作用机制.方法 成年雄性sD大鼠60只,随机分为正常对照组(C组),高温组(HT组),高温林格组(RL组),高温羟乙基淀粉林格组(HRL组),高温高渗氯化钠羟乙基淀粉组(HSH组),C组大鼠置:P(25-26)℃的环境中4 h,其余各组大鼠置于36 ℃具备生物氧供给的加温舱中3 h达到肛温(41.42)℃.加温开始30 min内根据实验分组输入相应的液体,3 h后出舱在室温下降温,加温期间监测肛温、平均动脉(mean arterial blood pressure,MAP)和动脉血气.采用伊文思兰(evans blue,EB)法检测大鼠血脑屏障的通透性,干湿法检测大鼠脑组织的百分含水量.结果 与C组相比,其他各组大鼠的脑百分含水量、肛温和EB值均有显著的升高(P<0.01).但HSH组升高值较其余3组为低(P<0.01).各实验组大鼠的MAP、pH、PaO2、Pa-CO2、Hct与C组相比差异有统计学意义(P<0.05).Na+、K+在HT组和HSH组明显升高(P

Effects of hypertonic sodium chloride hydroxyethyl strach 40 injection on the permeability of blood brain barrier in the whole-body hyperthermic rat's model

Objective To investigate the effects of hypertonic sodium chloride hydroxyethyl straeh 40 injection(HSH)on the permeability of blood brain barrier(BBB)in the whole-body hyperthermic(WBH)nat's model,and to explore the mecha-nism.Methods 60 adult male Sprauge-Dawley rats(220-250)g were randomized into 5 groups.Twelve rats were housed at a room with 25℃-26℃ temperature for 4h as control(C group).Other rats were exposed to WBH(36℃)to increase the rectal temperature to 41℃-42℃for 3h,thereafter were moved out of the heating container to let the temperature recover down for 1h.At WBH besinning,nothing(HT group),Ringer's solution(RL group),Haes+Ringer's solution(HRL group),or HSH (HSH group)was administered intravenously within 30 min(n=12).In the end of experiment,BBB permeability and brain wa-ter content were measured.Results After 3 h WBH,BBB and water content in WBH groups were signitlcandy higher than those in group C(P<0.01),with the lowest increasing extent in group HSH among test mts.MAP,blood pH,PaO2,PaCO2、and Hct significantiy changed in test groups(P