短时间缺血诱导亚铁血红素氧化酶-1对大鼠80%肝切除的影响

目的：观察短时间缺血对大鼠80％肝切除术后肝功能的影响，并对其作用机制进行探讨。方法：12只大鼠分成两组，缺血组肝右叶缺血15min,24h后行缺血肝叶亚铁血红素氧化酶－1免疫组化染色和ATP酶活性测定，同时行肝左叶和尾状叶切除，观察肝切除前，肝切除后1d,2d,3d和第5d肝功能指标GOT，GPT和LDH变化，第5d测定肝再生指数。结果：缺血组缺血后24h，肝细胞亚铁血红素氧化酶－1染色强阳性，并由中央小静脉周围向汇管区逐渐减弱，两组肝ATP酶活性无差异；80％肝切除后第5d,缺血组血清GOT，GPT和LDH明显低于对照组（P＜0．05），肝功能恢复较对照组快，两组肝再生指数无明显差异（P＞0．05），结论：肝缺血15min后24h，肝细胞亚铁血红素氧化酶－1被大量诱导产生，短时间缺血可以改善肝切除术后肝功能，缺血组肝切除术后肝功能的迅速改善可能与亚铁血红素氧化酶－1的诱生有关。

THE EFFECT OF A SHORT TIME ISCHEMIA INDUCING HEME OXYGENASE-1 ON THE LIVER FUNCTION AFTER 80% HEPATECTOMY IN RAT

Objective To observe the effect of a short time ischemia on the liver function after 80% hepatectomy in rats and to elucidate its mechanism.Methods 12 rats were divided into two groups,in the ischemia group,the right portal vein and hepatic artery were clamped 15 minutes,24 hours later,we detected the heme oxygenase-1(HO-1) expression by immunohistochemical staining and determined the ATPase activity in the ischemia lobe,and the left lobe and caudate lobe were cut off.We observed the fluctuation of the liver function before and after the operation.At the fifth day we determined the liver regeneration index.Results HO-1 was strongly detected in the pericentral liver cell 24 hours later after 15 minutes ischemia,no significant different was found between the two groups in ATPase activty (P>0.05).At the fifth day after 80% hepatectomy,the liver function indicator GPT.GOT and LDH improved significantly in the ischemia group(P<0.05),the liver regeneration index were the same in the two groups(P>0.05).Conclusion A large amount of HO-1 is induced 24 hours later after 15 minutes ischemia,a short time ischemia may improve the liver function after hepatectomy,the induced HO-1 may contribute to improvement of the liver function.