| 单克隆多克隆抗体不同标记ELISA技术对HBeAg/抗-HBe检测评估 |
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| 引用本文： | 高志良,姚集鲁. 单克隆多克隆抗体不同标记ELISA技术对HBeAg/抗-HBe检测评估[J]. 中山大学学报(医学科学版), 1993, 0(4) |
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| 作者姓名： | 高志良 姚集鲁 |
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| 作者单位： | 中山医科大学附属第三医院传染病科(高志良)，中山医科大学附属第三医院传染病科(姚集鲁) |
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| 摘 要： | 采用抗-HBe单克隆和多克隆抗体进行生物素或酶标记作酶免疫试剂检测HBeAg及抗-HBe,结果显示单克隆抗体(McAb)敏感性高于多克隆抗体(PcAb)。生物素试剂在检测HBeAg的稀释度为1:5000,比酶标法ELISA高1倍多,与RIA相当,且稳定性优于RIA。生物素标记抗体至少1年内保持效价不变,1年内批间变异系数为5.8％,相同标本重复检测变异系数为5.94％,是一种较理想的检测e系统的方法之一。另外,研究发现一孔一期法同时测HBeAg/抗-HBe时,无论标记抗体为单克隆或多克隆,其包被只能用单克隆抗体。
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| 关 键 词： | 生物素-亲和素系统 单克隆抗体 多克隆抗体 酶联免疫吸附试验 乙型肝炎病毒e抗原 乙型肝炎病毒e抗体 |
APPLICATION OF ELISA TECHNIQUES USING MONOCLONAL AND POLYCLONAL ANTIBODY REAGENTS LABELLING WITH BIOTIN FOR THE DETECTION OF HBeAg AND ANTI-HBe |
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| Gao Zhiliang Yao Jilu. APPLICATION OF ELISA TECHNIQUES USING MONOCLONAL AND POLYCLONAL ANTIBODY REAGENTS LABELLING WITH BIOTIN FOR THE DETECTION OF HBeAg AND ANTI-HBe[J]. Journal of Sun Yatsen University(Medical Sciences), 1993, 0(4) |
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| Authors: | Gao Zhiliang Yao Jilu |
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| Abstract: | Application of ELISA techniques using monoclonal and polyclonal anti-HBe reagents labelling with biotin was reported. Its sensitivity is similar to RIA and higher than Mc-ELISA. Anti-HBe conjugate is stable for at least one year. The succeed of single well and one step incubation techniques for the detection of HBeAg and anti-HBe depended on using McAb rather than PcAb for coating. Therefore, Mc-ABC-ELISA is the choice of method for the detection of HBeAg and anti-HBe. |
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| Keywords: | biotin-avidin system monoclonal antibody polyclonal antibody ELISA HBeAg anti-HBe |
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