长链非编码RNA SPATA3-AS1在胃癌中的表达及意义

目的: 研究长链非编码RNA(long non-coding RNA, lncRNA)SPATA3-AS1在胃癌患者癌组织和血浆中的表达及临床意义，并进一步探讨其对胃癌细胞株的影响及其潜在机制。方法：采用实时荧光定量PCR(qRT-PCR)检测80例胃癌及癌旁组织、29例胃癌患者和29例健康者血浆中SPATA3-AS1的表达水平；对胃癌患者SPATA3-AS1表达与其临床病理特征行统计学分析。敲减SPATA3-AS1，分别转染胃癌AGS、HGC-27细胞，采用细胞增殖、克隆形成、Transwell实验及流式细胞术分别检测细胞增殖、迁移、侵袭和凋亡；采用qRT-PCR检测肿瘤相关基因mRNA的表达水平。结果：SPATA3-AS1在胃癌组织中呈高表达，其表达与淋巴结转移、肿瘤TNM分期、肿瘤浸润深度及肿瘤预后相关；胃癌患者血浆中SPATA3-AS1也呈高表达，其表达与淋巴结转移、肿瘤直径、肿瘤TNM分期呈正相关。血浆中SPATA3-AS1表达水平诊断胃癌ROC曲线下面积为0.788。敲减SPATA3-AS1后，胃癌AGS、HGC-27细胞增殖及移动活力减弱，细胞凋亡能力增强。同时N-钙黏蛋白、Twist1、Snail mRNA表达下调而E-钙黏蛋白mRNA表达上调。结论：SPATA3-AS1在胃癌患者癌组织及血浆中呈高表达并与其预后相关；敲减SPATA3-AS1后胃癌细胞增殖、迁移及侵袭等能力均受到抑制，细胞凋亡增强，可能与对上皮间质转化过程的调控有关。

Expression of lncRNA SPATA3-AS1 in gastric cancer and its significance

Objective:  To investigate the expression of long non-coding RNA(lncRNA) SPATA3-AS1 in gastric cancer tissues and plasma and its clinical significance, and to further explore its effect on gastric cancer cells and its potential mechanism. Methods: Real-time quantitative fluorescence PCR(qRT-PCR) was used to detect the expression level of SPATA3-AS1 in plasma of 80 pairs of gastric cancer and adjacent tissues, 29 gastric cancer patients and 29 healthy subjects. The difference of SPATA3-AS1 expression and clinicopathological characteristics of gastric cancer patients were statistically analyzed. Cell proliferation, clone formation, transwell assay and flow cytometry were used to analyze the effects of SPATA3-AS1 knockdown on gastric cancer cells(AGC and HGC-27).The effect of SPATA3-AS1 knockdown on the expression level of tumor-related molecules was detected by qRT-PCR. Results: SPATA3-AS1 was highly expressed in gastric cancer tissues, and its expression level was correlated with lymph node metastasis, TNM stage, depth of tumor invasion and prognosis. Plasma SPATA3-AS1 was also highly expressed in patients with gastric cancer, and its expression was positively correlated with lymph node metastasis, tumor diameter, and TNM stage of gastric cancer. The area under the receiver operating characteristic curve (ROC curve) for the diagnosis of gastric cancer by the expression level of SPATA3-AS1 in plasma was 0.788. After SPATA3-AS1 knockdown, the proliferation and mobility of AGS and HGC-27 cells were decreased, and apoptosis was enhanced. Meanwhile, the mRNA expression of N-cadherin, Twist1 and Snail were down-regulated while the mRNA expression of E-cadherin was up-regulated in SPATA3-AS1 knockdown cells. Conclusion: SPATA3-AS1 is highly expressed in cancer tissues and plasma of patients with gastric cancer and is associated with prognosis. After SPATA3-AS1 knockdown, the proliferation, migration and invasion of gastric cancer cells were inhibited, and the cell apoptosis was enhanced, which may be related to the regulation of the process of epithelial-mesenchymal transformation. ［Key words］gastric cancer; long non coding RNA; SPATA3-AS1; epithelial-mesenchymal transition