在线、离线结合快速筛选杨梅叶中黄酮类抗氧化活性成分

目的:建立一种2,2-联苯基-1-苦基肼基高效液相色谱(2,2-diphenyl-1-picrylhydrazyl-high performance liquid chromatography,DPPH-HPLC)法在线筛选杨梅叶中黄酮类抗氧化活性成分,并对抗氧化活性进行检测和评价.方法:采用Shimadzu C18柱...

Rapid screening of flavonoids antioxidant components from Bayberry leaves by on-line and off-line assays

Objective: To establish a 2, 2-diphenyl-1-picrylhydrazyl-high performance liquid chromatography (DPPH-HPLC) method for screening the antioxidant active components of flavonoids in the Bayberry leaves, and to detect and analyze antioxidant active components. Methods: Flavonoids in Bayberry leaves were determined by high performance liquid chromatography (HPLC) with the Shimadzu C18 (150 mm×4.6 mm, 5 μm). Compounds with high antioxidant activity were screened by DPPH-HPLC, and their antioxidant activities were evaluated by 2, 2-al phenyl-1-bitter base hydrazine (DPPH), 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) experiments. Results: Four flavonoids including myricitrin, myricetin, quercetin and kaempferol were detected by HPLC and separated completely. All the four compounds had antioxidant activities, among which quercetin had the strongest antioxidant capacity, followed by myricetin, and both of them have stronger antioxidant capacity than L-ascorbic acid, while myricitrin and kaempferol have weaker antioxidant capacity. Conclusion: The established DPPH-HPLC method could screen the antioxidant active components in the complex system.