N-乙酰半胱氨酸对哮喘小鼠模型气道炎症和氧化应激的影响

目的:探讨N-乙酰半胱氨酸(N-acetylcysteine,NAC)对哮喘模型小鼠气道炎症和氧化应激反应的影响及可能的机制.方法:选择18只SPF级BALB/c雌性小鼠,随机均分为对照组、哮喘组、N-乙酰半胱氨酸组,每组6只.哮喘组、N-乙酰半胱氨酸组雾化激发构建小鼠哮喘模型,N-乙酰半胱氨酸组每次雾化开始前30 m...

Effects of N-acetylcysteine on airway inflammation and oxidative stress in asthma model mice

Objective: To investigate the effect of N-acetylcysteine (NAC) on airway inflammation, oxidative stress in asthma model mice and its potential mechanism. Methods: Eighteen female BALB/c mice were randomly divided into control group, asthma group, and N-acetylcysteine(NAC) group, with 6 in each group. Asthma group and NAC group were atomized to construct asthma model, and NAC group was given NAC (300 mg/kg) by intragastric instillation 30 min before atomization, and control group was treated with the same volume of PBS. Samples were harvested within 24 h after the end of nebulization. Collection of bronchoalveolar lavage fluid was used to count inflammatory cells. The levels of serum cytokines IL-13, IL-5 and IFN-γ were measured by ELISA. HE staining was used to observe the pathological changes of mice lung tissue, and inflammation score was performed. The levels of malondialdehyde and glutathione(GSH) in lung tissue were measured by colorimetry. The expression of nuclear factor erythroid 2 related factor 2(Nrf2) and heme oxygenase 1(HO-1) in lung tissue were measured by quantitative real-time PCR method. Results: Compared with the control group, there were lots of inflammatory cells infiltration in the lung tissue, the alveolar structure was obviously destroyed, the number of inflammatory cells, the levels of IL-5, IL-13, inflammation score, and the levels of malondialdehyde were significantly increased (all P<0.05), the levels of IFN-γ and GSH were significantly decreased(all P<0.05), the expression of Nrf2 mRNA and HO-1 mRNA showed no significant difference in asthma group. Compared with the asthma group, the infiltration of inflammatory cells in lung tissue and the destruction of alveolar structure were reduced, the number of inflammatory cells, IL-5, IL-13 contents, inflammation score, malondialdehyde contents were significantly decreased(all P<0.05), the contents of IFN-γ, GSH and the expression of Nrf2 mRNA and HO-1 mRNA were significantly increased in NAC group (all P<0.05). Conclusion: NAC could reduce airway inflammation and oxidative stress in asthmatic mice, which may be attributed to activation of Nrf2/HO-1 signaling pathway.