人子宫体部平滑肌收缩相关蛋白的筛选

目的：筛选与人子宫平滑肌收缩相关的蛋白质以探讨人子宫体部平滑肌收缩及分娩发动的分子机制。方法：应用双向凝胶电泳技术分离人足月自然临产和未临产子宫体部平滑肌组织总蛋白，经考马斯亮蓝染色、图像分析、识别差异表达的蛋白质点，基质辅助激光解吸电离飞行时间质谱得到相应的肽质量指纹图谱，搜索数据库鉴定部分差异表达蛋白质；Westernl印迹对3个差异表达蛋白进行验证。结果：建立了人足月自然临产和未临产子宫体部平滑肌的双向凝胶电泳图谱，质谱鉴定得到自然临产和未临产之间的表达差异大于2倍的蛋白质点20个，其功能涉及细胞骨架、钙结合力、分子伴侣、能量代谢、信号转导和抗氧化能力等；Westernl印迹验证结果与蛋白质组学结果一致。结论：筛选并鉴定了20个子宫体部平滑肌收缩相关蛋白质，提示细胞骨架改变、钙结合能力增强是分娩发动的重要原因。

Identification of contraction related proteins in corpus myometrium at labor

Objective： To explore the molecular mechanism of human corpus myometrium contraction related proteins and parturition. Methods： The proteins of human corpus of myometrium tissues from full term non-in labor （38- 41 weeks amenorrhea） and full term in labor （38-41weeks amenorrhea） gravidas were separated by 2-dimensional gel electrophoresis （2-GE）, respectively. Then gels were stained by Coomassie brilliant blue G250, scanned by Image scanner and analyzed with PDQuest software. The differentially expressed protein spots of corpus myometrium between the 2 groups were identified by peptide mass finger print based on matrix-assisted laser desorption/ionization time of flight massspectrometry （MALDI-TOF-MS） and database proteins were confirmed by Western blot. searching. Three identified differentially expressed Results： Well resolved and reproducible 2D-GE maps of human corpus myometrium from non-in labor and in-labor gravidas were acquired. Twenty more than 2-fold differentially expressed protein spots were identified by MALDI-TOF-MS. These proteins were involved in cell structure, calcium- binding, chaperone, energy metabolism, signal transduction, and antioxidant. Conclusion： Twenty contraction related proteins of human corpus myometrium have been identified, indicating that cell structure and calcium-binding are important reasons for the contraction of human corpus myometrium.