采用副结核杆菌建立肉芽肿性肠炎动物模型的研究

目的:利用副结核杆菌接种地鼠,以图建立一个模拟克隆病的小动物模型。方法:将40只地鼠随机分为两组,实验组:20只腹腔接种副结核杆菌;对照组:20只分别接种生理盐水和死菌(各10只)。8个月后处死动物,作病理学观察,并对动物肠组织进行 Polymerase chain reaction(PCR)扩增副结核杆菌 DNA。结果:实验组 80% (16/ 20)的地鼠出现肉芽肿性肠炎。实验组所有 20只地鼠的肠组织均扩增出 400 bp的 DNA 片段。结论:建立了一个新的肉芽肿性肠炎的小动物模型。

Study of Animal Model of Granulomatous Enteritis Induced by Mycobacterium Paratubercu-losis in Hamsters

Purpose: To establish a small animal model of granulomatous enteritis mimicing crohn' s disease by mycobacterium paratuberculosis(MP) in hamsters. Methods: Inoculum consisted of 10 mg freshly harvested MP. 40 hamsters were inoculated intraperitoneally, twenty received inoculum, ten received dead inoculum and ten received saline. Tissue was obtained from intestine and evaluated macroscopically and histologically, and detected by polymerase chain reaction(PCR) after 8 months postinoculation. Results: 16 of 20 hamsters received MP showed granulomatous changes and ulcerative formation, and 400 bp DNA fragment was ampliphied by PCR in 20 hamsters of test group. But the control hamsters didn't show gran-ulomatous enteritis. Conclusion: An animal model of granulomatous enteritis (mimicing CD) has been established by MP inoculated intraperitoneally.