肿瘤血管靶向肽GX1的受体筛选

目的：筛选肿瘤血管靶向肽GX1（CGNSNPKSC）的受体。方法：化学合成生物素标记的GX1,分离培养原代人脐静脉内皮细胞（HUVECs）,培养并收集共培养人脐静脉内皮细胞（co-HUVECs）全蛋白;应用免疫沉淀、免疫磁珠法分离富集与GX1结合的蛋白质;采用银染检测,确定并获取特异性蛋白条带;利用基质辅助激光解析电离飞行时间质谱（MALD I-TOF-MS）及生物信息学分析,对条带蛋白进行测序和分析。结果：免疫沉淀和银染法获得与GX1特异性结合的蛋白条带,其分子量在10-15KDa之间。利用MALDI-TOF-MS及生物信息学分析获得了一个有意义的候选蛋白核苷二磷酸激酶A（NDPKA）。结论：利用免疫沉淀-质谱法筛选获得了GX1受体的候选蛋白,为研究GX1的作用机制奠定了基础。

Screening of the receptors of peptide CGNSNPKSC

Objective： To screen the receptor of GX1（CGNSNPKSC） binding specifically to vascular endothelial cells in gastric cancer.Methods： We synthesized the biotin-labeled GX1 peptide and isolated and identified human umbilical vein endothelial cells（HUVECs）,collected the total cell proteins of co-cultrued human umbilical vein endothelial cells（co-HUVECs）.By using immunoprecipitation and immune magnetic beads separation to obtain proteins combined with GX1 peptide was screened.Then,the protein band was identified by Matrix-Assisted Laser Desorption/ Ionization Time of Flight Mass Spectrometry（MALDI-TOF-MS）,and analyzed with bioinformatics.Results： By using immunoprecipitation,one band in the position of 10-15 KDa was found to bind selectively to the GX1.Conclusion： By using immunoprecipitation and mass spectrometry,we found one candidate protein for receptor of GX1,which may provide foundation to the study of GX1.