非小细胞肺癌患者组织中的EGFR、K-Ras、ERCC1的表达及其预后分析

目的：探讨人表皮生长因子受体（EGFR）、Kirsten鼠肉瘤基因（K-ras）和核苷酸剪切修复复合体1（ERCC1）蛋白在非小细胞肺癌（NSCLC）组织和癌旁组织中的表达情况及其与NSCLC生理病理特征的相关性。方珐：用免疫组化方法检测NSCLC患者手术切除标本89例，其中取32例患者癌旁组织标本，检测各标本中EGFR、K—Ras和ERCC1蛋白的表达。结果：NSCLC组织中EGFR蛋白阳性表达率为52．8％，在癌旁组织中阳性表达率为3．12％；Ⅰ-Ⅱ期、无淋巴结转移患者中阳性表达率明显低于Ⅲ-Ⅳ期、有淋巴结转移患者；K—Ras蛋白阳性表达率为41．5％，在癌旁组织中为9．4％；腺癌表达率明显高于鳞癌；ERCC1蛋白阳性表达率为44．9％，在癌旁组织中为3．12％，差异均具有统计学意义（P〈0．05）。结论：NSCLC患者中EGFR、K—Ras和ERCC1蛋白的表达均为上调趋势，其表达是指导患者临床个体化疗方案及疗效和预后评价的重要指标。

Expression of EGFR,K-Ras and ERCC1 in non-small cell lung cancer and its prognosis

Objective： To detect expression of EGFR, K-Ras and ERCC1 protein in tissues of non-small cell lung cancer （NSCLC） and adjacent tissues and to analyze the correlation between the expression level and of pathologic parameters of the disease. Methods： expression of EGFR, K-Ras and ERCC1 protein in eighty-nine specimens of resected tissues of confirmed lung cancer and twenty-nine specimens of adjacent tissues of these lung cases were detected by immunohistochemical method. Results： Positive expression rate of EGFR protein in NSCLC tissues and adjacent tissues were 52.8% and 3.12%, respectively, EGFR expression rate in stage Ⅰ -Ⅱ NSCLC without lymph node metastasis were significant lower than that in stage Ⅲ-Ⅳ cases with lymph node metastasis. Positive expression rate of K-Ras protein was 41.5% in NSCLC tissues and was 9.4% in the adjacent tissues, and the expression rate was higher in adenocarcinoma than that in squamous cell carcinoma. Positive expression rate of ERCC1 protein was 44.9% in NSCLC tissues and was 3.12% in the adjacent tissues, the difference was significant （P〈0. 05）. Conclusion： The expression of EGFR,K-Ras and ERCC1 protein was up-regulated in NSCLC indicating they are important indicators for individual chemotherapy and evaluation of prognosis.