弓形虫感染者血清IgM和IgG抗体免疫印迹谱分析

目的探索近期和慢性弓形虫感染的免疫诊断标志物。方法采用无毒灵敏的TMB为底物的酶联免疫印迹技术,分析弓形虫感染者IgM或IgG抗体阳性血清与弓形虫速殖子可溶性抗原的免疫反应谱。结果IgM抗体对p35、p38和p22的识别比例达80%以上,且反应带出现率较其它抗原组分高,而p32、p30与IgG抗体的反应带出现率和识别比例也分别高达40%和80%以上。其中,反应最强烈最具代表性的分别属p35(出现率81.5%,P<0.0001)和p32(出现率57.1%,P<0.0001)。结论p35、p38、p22可能为近期弓形虫感染的诊断标志物,p32和p30则可能是慢性弓形虫感染的诊断标志物。

Analysis of immunoblot patterns of IgM and IgG antibodies in human sera of patients infected by Toxoplasma gondii

To explore the diagnostic markers of recent and chronic phases of Toxoplasma gondii infection.By using nonpoisonous tetramethylbenzidine (TMB) as horseradish peroxidase substrate for immunoblot, the patterns of IgM and IgG antibodies in human sera of patients infected by T. gondii reacted with soluble tachyzoite antigens were analyzed. The results showed that the frequencies of antigenic components of p35, p38 and p22 reacted with the IgM antibody were 81.5%, 44.4% and 40.7%,respectively and the recognition proportion of the components recognized by IgM antibody attained over 80%. The recognition frequencies and proportions of p32 and p30 recognized by IgG antibody were also over 40% and 80% respectively. It is concluded that p35, p38 and p22 may act as diagnostic markers of recent phase of Toxoplasma gondii infection , and p32 and p30 probably were the diagnosis markers.For chronic infection with T.gondii.