缺血预处理对胆管缺血再灌注损伤的保护作用

目的观察缺血预处理对胆管缺血再灌注损伤的保护作用。方法24只SD大鼠随机分为假手术（SO）组、缺血再灌注（IR）组、缺血预处理（IP）组，每组各8只。建立大鼠肝外胆道缺血再灌注模型，IP组于缺血再灌注前行一次缺血预处理。各组术毕取下腔静脉血、胆汁及肝外胆管标本。检测血中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平，胆汁中r-谷氨酰转肽酶（r-GT）活性，胆管组织中超氧化物歧化酶（SOD）水平；胆管组织电镜下行病理观察。结果IP和IR组的肝及胆管组织均受损伤，IR组较IP组严重。IR组SOD[（161．71±10．34）U／mgprot]显著低于SO组[（181．19±11．73）U／mgprot]和IP组[（175．23±6．48）U／mgprot]（P〈0．05），而ALT[（240．00±102．95）u／L]、AST[（558．31±236．01）U／L]及r-GT[（32．05±14．96）U／L]均显著高于SO组[（97．50±47．02）U／L]、[（239．65±91．72）U／L]、[（11．32±7．25）U／L]和IP组[（145．38±65．32）U／L]、[（372．70±152．59）U／L]．[（17．63±7．26）U／L]（P〈0．05）。病理观察IP纰亦优于IR组。结论IP对大鼠胆管IR损伤具有保护作用。

Effects of Ischemic Preconditioning on Ischemia-reperfusion Tissue of Bile Duct in Rats

Objective To observe the protective effects of ischemic preconditioning on injury of ischemia-reperfusion tissue of bile duct in rats.MethodsTwenty four SD rats were randomly divided into sham operation group,ischemic reperfusion group and ischemic preconditioning group.There were eight rats in each group.A rat model of acute extrahepatic bile duct ischemia-reperfusion was established.IP group was achieved with ischemic reperfusion before reinflue.The blood of rats were drew and then levels of ALT and AST i...