HPLC法测定穿心莲滴丸中穿心莲内酯和脱水穿心莲内酯的含量

目的建立测定穿心莲内酯和脱水穿心莲内酯总含量的方法,用于穿心莲滴丸的质量控制。方法采用HPLC法。色谱柱:Diamonsil ODS柱(4.6 mm×250 mm,5μm),流动相:甲醇-水(体积比60∶40),流速:1.0 mL.min-1,检测波长:231 nm,柱温:35℃。结果穿心莲滴丸中穿心莲内酯、脱水穿心莲内酯与其他成分分离良好;穿心莲内酯的质量浓度在50.0~300.0 mg.L-1(r=0.999 8)内、脱水穿心莲内酯的质量浓度在20.0~120.0 mg.L-1(r=0.999 7)内与峰面积呈现良好的线性关系;穿心莲内酯、脱水穿心莲内酯的回收率分别为101.62%、97.72%,RSD分别为1.9%、2.1%。结论该方法可有效地控制穿心莲滴丸的质量。

Determination of andrographalide and dehydroandrogrepjolide in Chuanxinlian pills by HPLC

Objective To establish a determination method of andrographalide and dehydroandrogrepjolide in Chuanxinlian pills. Methods HPLC was carried out with C₁₈ column with methanol-water (V:V=60:40) as the mobile phase, the detection wavelength was at 231nm, the column temperature was 35℃, the flow rate was 1.0 mL&;#8226;min^-1. Results The average recovery of andrographalide for the added samples was 101.62% with RSD of 1.92%; the average recovery of dehydroandrogrepjolide for the added samples was 97.72% with RSD of 2.12%. Conclusions The experiment shows that this method is simple, accurate with good reproducibility and can be used in quality control of Chuanxinlian pills.