宫颈癌癌前病变及早期癌组织中微淋巴系统的定量观察

目的:探讨宫颈癌前病变和宫颈癌组织中微淋巴管与宫颈癌发生、发展及转移侵袭的关系。方法:部分宫颈组织流式细胞仪检测LYVE-1阳性细胞和Prox-1阳性细胞。46例宫颈组织采用LYVE-1和Prox-1相关抗原免疫组化染色,统计微淋巴管密度及其面积百分比,结合临床资料进行分析。结果:(1)流式细胞检测宫颈组织中存在LY-VE-1(+)和Prox-1(+)细胞;(2)癌前病变LMVD(16.13±10.96)条/4HP,LVA(2.00±1.51)%与Ⅰ期宫颈癌LMVD(24.4±11.39)条/4HP,LVA(2.10±1.61)%相比无统计学差异(P>0.05),与Ⅱ期宫颈癌(28.58±12.29)条/4HP(P<0.01),LVA(3.12±1.77)%相比有显著差异(P<0.01);(3)有淋巴转移者LMVD为(29±11.42)条/4HP,LVA为(3.30±1.76)%相对无转移者(20.23±11.06)条/4HP(2.07±1.14)%均有显著差异(P<0.01);(4)细胞HG1级者LMVD(17.19±9.08)条/4HP,LVA(2.20±1.53)%相对HG3(37.43±8.54)条/4HPLVA(2.85±2.23)%有显著差异(P<0.05);(5)LMVD与LVA与病理类型和月经状态无明显相关。结论:宫颈癌前病变和早期癌组织中高淋巴管分布与淋巴转移,肿瘤病理分期,肿瘤细胞分化程度有关。

A quantitative analysis of lymphatic microvessels in human cervix neoplasm

Objective:To study the role of lymphatic microvessels in cervical neoplasm and its relationship with tumor development and metastasis.Methods:Monoclonal lymphatic vessel endothelial HA receptor- 1(LYVE-1) and Prox-1 antibodies were used to earmark cervical tissue single cell suspension for FCM detection.These antibodies were also used to immunostain the lymphatic microvessels in the paraffin sections of cervical neoplasm tissues of 46 cases.Computer-assisted morphometric analysis was used to quantitatively analyse lymphatic microvessel density (LMVD) and lymphatic vessel area (LVA).Results:(1)There were LYVE-1(+)and Prox-1(+)cells in cervical tissues;(2)The LMVD (16.13±10.96)vessels/4HP and LVA (2.00±1.51)% of precancerous,both were significantly fewer than that in stage Ⅰ(24.4±11.39)vessels/4HP and(2.10±1.61)%(P<0.05)]and stage Ⅱ(28.58±12.29)vessles/4HP,(2.10±1.61)%(P<0.01)];(3)LMVD (29±11.42) vessels/4HP and LVA(3.3±1.76)% in patients with lymph node metastasis were all higher than those of the patients without lymph node metastasis (20.23±11.06)vessels/4HP and (2.07±1.14)%,P<0.01];(4)LMVD of histological gradeⅠLMVD(17.1±9.08)vessels/4HP,LVA (2.20±1.53)% were both lower than Ⅲ (37.43±8.54)vessels/4HP and (2.85±2.23)% (P<0.05) (5) There were no notability relationship between LMVD or LVA and histological classification or menopause.Conclusion:There was correlation between higher lymphangiogenesis in precancerous lesion and early cervical neoplasm and lymphatic vessel invasion,pathological stages of tumor and histological grade of tumor cells.