黄花石蒜不定根的离体诱导与培养研究

目的摸索黄花石蒜不定根的离体诱导与培养条件,并对其有效成分加兰他敏进行初步检测。方法以黄花石蒜双鳞片为外植体,接种于不同培养基上,观察长根情况;以离体培养的不定根为材料,检测其是否含有有效成分加兰他敏。结果双鳞片在MS 2,4-D2mg/L培养基中能诱导出愈伤组织;愈伤组织在MS IBA1mg/L BA0.1 mg/L中诱导生根最佳;薄层色谱显示黄花石蒜不定根的培养品中含加兰他敏。结论离体培养黄花石蒜的不定根,可能成为加兰他敏的一个新来源。

Study of Lycoris aurea on induction and culture of adventitious root in vitro

Objective To investigate the induction and culturing condition of adventitious root of Lycoris aurea in vitro,and detect its active principle galanthamine.Methods The twin-squamas of Lycoris aurea was took as explant to be cultured in different mediums.The active galanthamine was detected in the adventitious roots.Results The callus could induce directly from twin-squamas in MS 2,4-D 2.0 mg/L;the callus in medium of IBA 1 mg/L BA 0.1 mg/L was the best condition to induce rooting;and the activity-galanthamine detected with TLC was contained in the roots.Conclusions The adventitious root will be a new resource to obtain the galanthamine from culturing Lycoris aurea in vitro.