前S1抗原与HBV血清标志物及HBV-DNA相关性的探讨

目的探讨前S1抗原(PreS1)与HBV血清标志物及HBV-DNA相关性。方法对102例慢性乙型肝炎患者和73例健康人采用酶联免疫吸附试验(ELISA)检测HBV血清标志物和PreS1及聚合酶链反应(PCR)方法检测HBV-DNA。结果102例慢性乙型肝炎患者中,PreS1和HBeAg与HBV-DNA的总符合率分别为70.6%和75.5%;PreS1的敏感性高于HBeAg,但特异性则相反;HBeAg阴性患者部分仍存在着病毒复制;随着HBV-DNA拷贝数的升高,PreS1和HBeAg的阳性率亦随之升高;在HBV-DNA低拷贝数时PreS1的阳性率明显高于HBeAg。结论PreS1的检测可以较好地反映HBV存在和复制的情况,PreS1作为辅助或补充指标联合HBV血清标志物与HBV-DNA同步动态监测,具有重要的临床应用价值。

Relativity among PreS1-antigen, HBV Markers and HBV-DNA

OBJECTIVE To study the relativity among PreS1-Antigen,HBV markers and HBV-DNA. METHODS The HBV markers,PreS1-antigen and HBV-DNA were determined by ELISA and PCR in 102 patients with chronic hepatitis B and 73 healthy persons. RESULTS Among 102 patients with chronic hepatitis B,the concordance rate of PreS1-antigen and HBeAg with HBV-DNA was 70.6% and 75.5%.The sensitivity of PreS1 was better than HBeAg but the specificity was contrary.It represented some patients with HBeAg(-) still had viral replication.On the increase in the level of HBV-DNA,the positive rates of PreS1-antigen and HBV markers increased. CONCLUSIONS The detection of PreS1-antigen can well reflect the HBV replication.The synchronous dynamic detection of PreS1-Antigen,HBV markers and HBV-DNA has its important clinical meaning.