| HPLC法测定腰痛丸中补骨脂素和异补骨脂素的含量 |
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| 引用本文： | 许菊. HPLC法测定腰痛丸中补骨脂素和异补骨脂素的含量[J]. 中国药师, 2009, 12(8): 1077-1079 |
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| 作者姓名： | 许菊 |
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| 作者单位： | 绍兴市药品检验所,浙江绍兴312000 |
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| 摘 要： | 目的：建立腰痛丸中补骨脂素和异补骨脂素含量测定HPLC方法。色谱柱：ZORBAX SB—C18（250mm×4．6mm，5μm），流动相：甲醇-水（55：45），流速1．0ml·min^-1，检测波长：246nm。结果：补骨脂素和畀补骨脂素分别在0．05～0．29μg（r=0．9999）和0．05～0．27μg（r=0．9998）范围内线性关系良好，回收率分别为96．0％（RSD=1．5％）和98．6％（RSD=1．6％，n=6）。结论：方法简便、快速，可用于腰痛丸的含量测定。
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| 关 键 词： | 腰痛丸 补骨脂素 异补骨脂素 高效液相色谱法 |
Determination of Psoralen and Isopsoralen in Yaotong Pills by HPLC |
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| Xu Ju. Determination of Psoralen and Isopsoralen in Yaotong Pills by HPLC[J]. China Pharmacist, 2009, 12(8): 1077-1079 |
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| Authors: | Xu Ju |
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| Affiliation: | Xu Ju ( Shaoxing Institute for Drug Control , Zhejiang, Shaoxing 312000, China) |
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| Abstract: | Objective： To establish a method of determination of psoralen and isopsoralen in Yaotong pills by HPLC. Method： The ZORBAX SB-C18 column（ 250mm ×4. 6mm,5μm）was used, the mobile phase was methanol-water （55： 45）, the detection wavelength was 246nm the flow rate was 1.0ml · min ^-1 Result： The linear ranges of psoralen and isopsoralen were 0. 05-0. 29μg（ r = 0. 999 9 ）, and 0.05 4）. 27 μg（ r = 0. 999 8 ） , the average recoveries were 96.0% （ RSD 1.5% ） and 98.6% （ RSD1. 6% ）, （ n = 6 ） respectively. Conclusion：The method is simple and fast, it can be used for the determination of psoralen and isopsoralen in Yaotong pills. |
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| Keywords: | Yaotong pills Psoralen Isopsoralen HPLC |
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