p53蛋白在鼻咽癌中的表达及其临床意义

目的：从基因水平探讨抑癌基因ｐ５３蛋白与鼻咽癌的关系。方法：本文采用Ｗｗｓｔｅｒｍ ｂｌｏｔ结合非放射性免疫沉淀技术，对鼻咽癌细胞株、鼻咽癌活检组织和鼻咽粘膜慢性炎症组织进行了ｐ５３蛋白表达的研究。结果：７株鼻咽癌细胞株均发现ｐ５３蛋白表达异常条带。１０４例鼻咽癌活检组织中有１２例出现ｐ５３蛋白表达异常，检出率为１１．５４％。８例鼻咽慢性炎症活检组织中ｐ５３蛋白表达未见异常。１２例ｐ５３蛋白表达异

p53 Protein Expression and Its Clinical Significance in HumanNasopharyngeal Carcinoma

Objective: To study the relationship between p53 protein expression and progression of nasopharyngeal carcinoma. Methods: Human nasopharyngeal carcinoma cell line, primary nasopharyngeal carcinoma and chronic nasopharyngeal inflammation biopsy were examined for p53 protein expression by Western blot and immunoprecipitation techniques. Results:Abnormal expression of p53 protein was seen in 7 nasopharyngeal carcinoma cell lines (CNE1 , CNE2-Z, HNE1, HNE2, HNE3, SUNE1 and HK1). In the 104 nasopharyngeal carcinoma biopsies, 12 (11.54%) showed abnormal expression of p53 protein. Abnormal expression of p53 was not found in 8 biopsies of the nasopharyngeal chronic inflammation. Two recurrences were found in 12 patients with p53 protein abnormal expression. Conclusion: p53 protein expression may play a role in the occurrence and development of nasopharyngeal carcinoma.