| 大肠癌及癌旁组织中端粒酶活性的检测及临床意义 |
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| 引用本文： | 张登学,牟永善,顾华丽. 大肠癌及癌旁组织中端粒酶活性的检测及临床意义[J]. 中国肿瘤临床, 1999, 26(10): 756-758 |
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| 作者姓名： | 张登学 牟永善 顾华丽 |
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| 作者单位： | 青岛医学院附属医院!山东省青岛市266003(张登学，牟永善，顾华丽，唐晓燕)，中国医学科学院肿瘤研究所(刘海灵) |
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| 基金项目： | 青岛市科学技术委员会基金!资助（97－ 10） |
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| 摘 要： | 目的：建立测定端粒酶活性的ＰＣＲ－ＴＲＡＰ方法并探讨其在大肠癌诊断中的意义。方法：应用端粒酶活性的ＰＣＲ－ＴＲＡＰ方法，对４０例大肠癌及其癌旁组织进行了检测。结果：４０例大肠癌组织中有３７例端粒酶活性呈阳性，阳性检出率为９１．７５％，癌旁组织中有２例阳性，阳性检出率为５．００％，两者相比差异有极显著性（Ｐ〈０．００１）；４０例大肠癌均为腺细胞癌，其中１１例伴随淋巴结转移的标本中１０例检测出活性，而
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| 关 键 词： | 肠肿瘤 端粒酶 活性检测 |
Detection of Telomerase Activity in Colorectal Cancer andIts Adjacent Tissues |
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| Zhang Dengxue Mu Yongshan Gu Huali et alAffiliated Hospital of Qingdao Medical University,Qingdao. Detection of Telomerase Activity in Colorectal Cancer andIts Adjacent Tissues[J]. Chinese Journal of Clinical Oncology, 1999, 26(10): 756-758 |
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| Authors: | Zhang Dengxue Mu Yongshan Gu Huali et alAffiliated Hospital of Qingdao Medical University Qingdao |
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| Abstract: | Objective: To evaluate the diagnostic value of telomerase activity in patients with colorectal cancer. Methods: Telomerase activity in colorectal cancer and its adjacent tissues of 40 patients were detected by using of PCR-TRAP method. Results: The positive rates of telomerase activity in the specimens of colorectal cancer and its adjacent tissues were 91.75%and 5.00%respectively (P< 0.001). Meanwhile, telomerase activity was not related with the differentiation type of the tumor and metastasis. Conclusion: It is suggested that PCR-TRAP is a valuable method for assaying activity of telomerase. Telomerase activity is related to colorectal cancer and it may be a diagnostic marker especially in early stage. |
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| Keywords: | Human colorectal carcinoma Telomerase Detection |
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