VEGF-siRNA对人舌癌TCA8113细胞体内及体外生长影响的初步研究

目的探讨针对血管内皮生长因子(VEGF)的小分子RNA干扰(siRNA)对舌癌TCA8113细胞体内和体外生长的影响。方法将两对特异性针对VEGF的siRNA真核表达载体,以空载体组做实验对照组,经脂质体转染人舌癌TCA8113细胞,经G418筛选后获得稳定表达,以未转染舌癌细胞作空白对照组,MTT检测转染后各组细胞增殖情况;将稳定转染的各组细胞接种裸鼠,接种后7 d开始,每4 d测量瘤体积,绘制肿瘤生长曲线,比较各时间段肿瘤生长,接种后47 d处死,比较瘤体积和瘤重。结果与实验对照组和空白对照组相比较,两个实验组细胞生长速度降低,细胞抑制率均显著增高,差异有统计学意义(P<0.05),裸鼠接种肿瘤生长缓慢,处死后测量到瘤体体积小、重量轻(P<0.05),而实验对照组和空白对照组细胞抑制率、瘤体体积、瘤重等差异无统计学意义(P>0.05)。结论VEGF-siRNA在体内和体外能有效的抑制人舌癌细胞的生长。

Effects of VEGF-siRNA on the growth of TCA8113 cell line of human tongue cancer in vitro and in vivo

Objective To access the influence of vector-based small interfering RNA(siRNA) targeting vascular endothelial growth factor(VEGF),on the growth of human tongue carcinoma cell line(TCA8113) in vitro and in vivo.Methods Two siRNA targeting VEGF constructed in eukaryotic expression vector(PU-VEGF-siRNA1,PU-VEGF-siRNA2),and vector without siRNA as experiment control,were transfected into TCA8113 cells by lipofectamine 2000,respectively.The non-transfected TCA8113 cell was used as negative control.The cell proliferation was detected by MTT.Twenty nude-mice were divided into four groups randomly,and then the transfected cells and non-transfected cell were subcutaneous injected on the back of mice,respectively.The tumor volume,weight,and growth curve of every group were compared.Results Compared with experiment and negative controls,cell proliferation was decreasend(P<0.05),the growth inhabiting rate was risedn(P<0.05) in vitro;the growth of tumors was significantly reduced,and tumor volume and weight decreased(P<0.05),in two experiment groups.But there were no significant difference of those between two controls(P>0.05).Conclusion VEGF-siRNA can suppress the TCA8113 growth effectively both in vitro and in vivo.