血小板活化因子在烧伤合并内毒素血症早期肺损伤中的作 …

目的 探讨血小板活化因子（ＰＡＦ）肿瘤坏死因子（ＴＮＦ）等在烧伤合并内毒素血症早期肺损伤中的作用及意义。方法 采用２０％ＴＢＳＡⅢ度烧伤合并内毒素注射复制大鼠早期肺损伤模型，检测血浆ＰＡＦ、ＴＮＦ含量等，观察肺组织病理形态学变化并应用ＰＡＦ受体拮抗剂防治早期肺损伤。结果 ＰＡＦ是烧伤合并内毒素血症早期先于ＴＮＦ出现变化的炎症介质，血浆ＰＡＦ浓度与早期肺操作程度呈正相关，注射ＰＡＦ受体拮抗剂ＢＮ５０

The role and significance of PAF in the early pulmonary injury following burns combined with endotoxemia in rats]

OBJECTIVE: To investigate the role and significance of PAF in the early pulmonary injury following burns combined with endotoxemia in rats. METHODS: An animal model, in which rats were subjected to 20% TBSA III degree burns combined with intraperitoneal injection of lipopolysaccharide (LPS), was used for this experiment. The changes in the pulmonary morphology were observed by LM, EM and immunohistochemistry(IHC). The contents of PAF and TNF-alpha in the plasma were determined with quantitative analysis. PAF receptor antagonist(BN50739) was used to treat early pulmonary injury. RESULTS: PAF was one of the inflammatory mediators to precede TNF changes during burns combined with endotoxemia in the early stage of pulmonary injury. The contents of PAF in the plasma was positively correlated to the extent of pulmonary injury, which may be alleviated after injection of PAF receptor antagonist (BN50739). CONCLUSION: The results suggest that PAF plays an very important pathogenic role in the early lung injury resulted from burns combined with endotoxemia.