龈沟液蛋白质成分的电泳分析

目的筛选出与疾病相关的蛋白质分子。方法采用龈沟冲洗法获取龈沟液，用微量自动快速电泳仪分析了８６例龈沟液样本（其中健康龈２０例、龈炎２４例、牙周炎４２例）。十二烷基磺酸钠－聚丙烯酰胺电泳分离的蛋白质区带用快速图象分析仪进行测量。结果在炎症情况下分子量为８２０００、７７０００及１７０００的血清源蛋白质发生率较健康龈升高，分子量为４１０００、２１０００、１２０００的非血清源蛋白质发生率亦较健康龈高，丰度值大。结论分子量为８２０００、４１０００、２１０００及１２０００的蛋白质可能与牙周炎有较密切的关系。

Electrophoretic analysis of protein composition in gingival crevicular fluid

Objective To seek the protein composition related to periodontal diseases. Methods 86 gingival crevicular fluid (GCF) samples were collected by rinsing the sulgingival sulci from subjects with healthy gingiva (20), gingivitis (24) and periodontitis (42). The protein composition were examined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS PAGE) on a Phast System. The gels were scanned and analyzed using Phast Image with computer. Results In inflammatory condition, the serumderived proteins with MW 82 000, 77 000, 17 000 occurred more frequently than in healthy gingiva, and the occurrence and abundance of non serum derived proteins with MW 41 000, 21 000, 12 000 were also increased in inflammatory condition compared with healthy gingiva. Conclusion The high levels of proteins with MW 82 000, 41 000, 21 000, 12 000 are close related to the periodontal inflammatory status.