益母草注射液对小鼠及大鼠实验性脑缺血的保护作用

目的 研究益母草注射液对小鼠及大鼠实验性脑缺血的保护作用及机制.方法 分别采用小鼠双侧颈总动脉结扎所致急性脑缺血模型、氰化钾致小鼠急性脑缺血模型及大鼠缺血再灌注损伤(MCAO)模型,观察益母草注射液作用于小鼠双侧颈总动脉结扎所致急性脑缺血后的脑指数、氰化钾致小鼠急性脑缺血后的翻正反射消失时间及大鼠MCAO后脑组织内丙二醛含量、超氧化物歧化酶及乳酸脱氢酶活力.结果 益母草注射液可降低双侧颈总动脉结扎所致急性脑缺血小鼠的脑指数,缩短氰化钾致小鼠急性脑缺血后的翻正反射消失时间,降低MCAO大鼠脑组织内丙二醛含量、提高超氧化物歧化酶及乳酸脱氢酶活力.结论 益母草注射液对小鼠及大鼠实验性脑缺血具有明显保护作用,为其临床应用提供了直接基础研究资料.

Protective effect of Yimucao (Herba leonuri) injection against cerebral ischemia: an experimental study in mice and rats

Objective To investigate the protective effect and mechanism of Yimucao(Herba leonuri)injection against experimental cerebral ischemia.Methods Mouse models of cerebral ischemia induced by bilateral carotid artery occlusion or potassium cyanide and rat models of middle cerebral artery occlusion(MCAO)-induced cerebral ischemia/reperfusion injury were established to evaluate the protective effect of Yimucao injection by measuring the changes in cerebral malondialdehyde(MDA)content and the activities of superoxide dismutase(SOD)and lactate dehydrogenase(LDH)after the injections.Results Yimucao injection significantly lowered the cerebral index of mice with cerebral ischemia,prolonged the survival time of mice poisoned with potassium cyanide,resulting also in significantly decreased MDA content and increased activities of SOD and LDH in the brain tissue of rats after a 10-min cerebral ischemia followed by 30 min of reperfusion.Conclusion Yimucao injection provides protective effect against experimental cerebral ischemia.