胚胎干细胞的鉴定方法

目的:胚胎干细胞具有全能性及核型正常的特征是其作为研究哺乳动物胚胎发育、细胞分化、外源基因的表达、以及利用基因突变的胚胎干细胞建立人类遗传病动物模型的理论基础。因而,胚胎干细胞的鉴定工作对于研究和利用胚胎干细胞具有重大的意义。资料来源:应用计算机检索Medline数据库1980-01/2003-12相关胚胎干细胞的文章,检索词“embryonicstemcell,embryo,Alkalinephos-phatase,Oct-4”,限定文章语言种类为英文。同时计算机检索中国期刊全文数据库1980-01/2003-12相关胚胎干细胞的文章,检索词“胚胎干细胞”,限定文章语言种类为中文。资料选择:对资料进行初审。纳入标准:与胚胎干细胞鉴定有关的文献。排除标准:文献中重复研究、综述类文章。资料提炼:主要收集了16篇有关胚胎干细胞鉴定的文献。查找全文。资料综合:将所选的资料按以下顺序归纳总结:①形态和生长特性的初步鉴定。②免疫学方法鉴定。③染色体相关鉴定。④全能性和多能性鉴定。结论:胚胎干细胞的鉴定并不是用某一种方法就可以的,而是一个鉴定过程。在这个过程中,我们应先检查碱性磷酸酶和核型,然后鉴定干细胞的表面标志物,当胚胎干细胞达到一定数量后再鉴定干细胞的发育全能性,每种鉴定都要重复多次。

Identification methods of embryonic stem cells

OBJECTIVE: Embryonic Stem Cell (ES) are characterized by totipotency and normal karyotype and provide the theoretical base in the following fields: embryonic developmeut of mammalian, cell differentiation, expres sion of exogenous genes and utilization of Ess to develop animal model for human inherited diseases. The identification of ES therefore is very important to research and utilize ES.DATA SOURCES: The relevant articles to embryonic stem cell between January 1980 and December 2003 were computer searched for in Medline with the key words "embryonic stem cell, embryo, Alkaline phosphatase,Oct-4"in English. Similarly, the relevant articles to embryonic stem cell between January 1980 and December 2003 were computer searched for in China Journal Full-text Database (CJFD) with the key word "embryonic stem cell" in Chinese.STUDY SELECTION: The articles were browsed firstly. The inclusion criteria were for those articles about the identification of embryonic stem cells. The exclusion criteria were for those about repetitive studies, reviews and other similar articles.DATA EXTRACTION: 16 articles about the identification of embryonic stem cells were collected. Then, the full-texts of the articles were looked through.DATA SYNTHESIS: The selected data were summarized in the following order: ①Preliminary identification of Ess based on morphology and growth;②Immunological valuation; ③Chromosome related identification; ④Identi fication of totipotency and pluripotency.CONCLUSION: The identification of embryonic stem cells is not the result of only one identifying method, but a process of identification. During this process of comprehensive identification, it is recommend to conduct AKP test firstly, karyotype analysis secondly, then examination of surface markers and finally identification of Ess totipotency when Ess are sufficient, takingcare to repeat every identification.